New two-photon fluorescent probe for multiphoton microscopy in biological media

R. Gvishi, G. Berkovic, Z. Kotler, P. Krief, J. Y. Becker, M. Sigalov, L. Shapiro, V. Khodorkovsky

Research output: Contribution to journalConference articlepeer-review

4 Scopus citations


An important ingredient in improving Multi Photon Laser Scanning Microscopy, MPLSM, is the development of efficient two-photon fluorescent (TPF) probes. We previously reported on a new class of TPF probes, specifically designed in order to maximize their efficiency in potential MPLSM applications. The fluorophores are based on a tetraketo derivative (TK) with a symmetric structure Donor-Acceptor-Donor (D-A-D). Those fluorophores have the following properties: a) Very large two-photon absorption coefficients (δ ∼ 1000GM); b) Two-photon excitation (TPE) peak wavelength strongly shifted to the red (λ ∼ 1μm); c) High fluorescence quantum efficiency; d) Large Stokes shifts of the fluorescence bands. We extended our work to a new fluorophore from this class that is more suitable for biological settings. This new fluorophore has a structure of crown-TK-crown that incorporates the ability to trap metal ions such as calcium. The TPE wavelength dependence of the TK-crown derivative is very similar to its analogous linear derivative with enhancement in the value of the cross-section, due to the stronger donor moieties. The TPE cross-section for the TK-crown derivative was about δ = 950 GM at λmax = 980 nm.

Original languageEnglish
Pages (from-to)437-442
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2003
EventPhotonics, Devices, and Systems II - Prague, Czech Republic
Duration: 26 May 200229 May 2002


  • Biological probes
  • Conjugated molecules
  • D-A-D
  • Metal ion caging
  • Multiphoton laser scanning microscopy (MPLSM)
  • Two-photon fluorophores

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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