NGF promotes amyloid precursor protein secretion via muscarinic receptor activation

Rachel Haring; David Gurwitz; Jacob Barg; Ronit Pinkas-Kramarski; Eliahu Heldman; Zipora Pittel; Haim D. Danenberg; Ada Wengier; Hairn Meshulam; Daniele Marciano; Yishai Karton; Abraham Fisher

doi:10.1006/bbrc.1995.2092

NGF promotes amyloid precursor protein secretion via muscarinic receptor activation

Rachel Haring, David Gurwitz, Jacob Barg, Ronit Pinkas-Kramarski, Eliahu Heldman, Zipora Pittel, Haim D. Danenberg, Ada Wengier, Hairn Meshulam, Daniele Marciano, Yishai Karton, Abraham Fisher

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Processing of β-amyloid precursor protein (APP) is coupled to several neurotransmitter receptors, including m1 muscarinic (m1AChR), and is associated with decreased amyloid deposition. Muscarinic agonist-stimulated APP secretion and membrane APP were measured in control and in NGF-differentiated PC12 cells stably transfected with m1AChR. This secretion was markedly enhanced following treatment with 50 ng/ml NGF for 3 days, and was observed using either carbachol or the M1-selective agonist AF102B. The effects of NGF were reflected by larger reductions in membrane-associated APP levels following muscarinic stimulation. These observations imply that M1 muscarinic receptors may act in concert with NGF to boost APP processing, and M1-selective agonists may thus be beneficial for reducing amyloid deposition by NGF-responsive neurons.

Original language	English
Pages (from-to)	15-23
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	213
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1995.2092
State	Published - 1 Jan 1995
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "NGF promotes amyloid precursor protein secretion via muscarinic receptor activation",

abstract = "Processing of β-amyloid precursor protein (APP) is coupled to several neurotransmitter receptors, including m1 muscarinic (m1AChR), and is associated with decreased amyloid deposition. Muscarinic agonist-stimulated APP secretion and membrane APP were measured in control and in NGF-differentiated PC12 cells stably transfected with m1AChR. This secretion was markedly enhanced following treatment with 50 ng/ml NGF for 3 days, and was observed using either carbachol or the M1-selective agonist AF102B. The effects of NGF were reflected by larger reductions in membrane-associated APP levels following muscarinic stimulation. These observations imply that M1 muscarinic receptors may act in concert with NGF to boost APP processing, and M1-selective agonists may thus be beneficial for reducing amyloid deposition by NGF-responsive neurons.",

author = "Rachel Haring and David Gurwitz and Jacob Barg and Ronit Pinkas-Kramarski and Eliahu Heldman and Zipora Pittel and Danenberg, {Haim D.} and Ada Wengier and Hairn Meshulam and Daniele Marciano and Yishai Karton and Abraham Fisher",

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doi = "10.1006/bbrc.1995.2092",

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T1 - NGF promotes amyloid precursor protein secretion via muscarinic receptor activation

AU - Haring, Rachel

AU - Gurwitz, David

AU - Barg, Jacob

AU - Pinkas-Kramarski, Ronit

AU - Heldman, Eliahu

AU - Pittel, Zipora

AU - Danenberg, Haim D.

AU - Wengier, Ada

AU - Meshulam, Hairn

AU - Marciano, Daniele

AU - Karton, Yishai

AU - Fisher, Abraham

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Processing of β-amyloid precursor protein (APP) is coupled to several neurotransmitter receptors, including m1 muscarinic (m1AChR), and is associated with decreased amyloid deposition. Muscarinic agonist-stimulated APP secretion and membrane APP were measured in control and in NGF-differentiated PC12 cells stably transfected with m1AChR. This secretion was markedly enhanced following treatment with 50 ng/ml NGF for 3 days, and was observed using either carbachol or the M1-selective agonist AF102B. The effects of NGF were reflected by larger reductions in membrane-associated APP levels following muscarinic stimulation. These observations imply that M1 muscarinic receptors may act in concert with NGF to boost APP processing, and M1-selective agonists may thus be beneficial for reducing amyloid deposition by NGF-responsive neurons.

AB - Processing of β-amyloid precursor protein (APP) is coupled to several neurotransmitter receptors, including m1 muscarinic (m1AChR), and is associated with decreased amyloid deposition. Muscarinic agonist-stimulated APP secretion and membrane APP were measured in control and in NGF-differentiated PC12 cells stably transfected with m1AChR. This secretion was markedly enhanced following treatment with 50 ng/ml NGF for 3 days, and was observed using either carbachol or the M1-selective agonist AF102B. The effects of NGF were reflected by larger reductions in membrane-associated APP levels following muscarinic stimulation. These observations imply that M1 muscarinic receptors may act in concert with NGF to boost APP processing, and M1-selective agonists may thus be beneficial for reducing amyloid deposition by NGF-responsive neurons.

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JO - Biochemical and Biophysical Research Communications

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NGF promotes amyloid precursor protein secretion via muscarinic receptor activation

Abstract

ASJC Scopus subject areas

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