TY - JOUR
T1 - Nitric Oxide Sensing through Azo-Dye Formation on Carbon Dots
AU - Bhattacharya, Sagarika
AU - Sarkar, Rhitajit
AU - Chakraborty, Biswarup
AU - Porgador, Angel
AU - Jelinek, Raz
N1 - Funding Information:
Financial assistance from the Ministry of Science, grant number 2015-243 is acknowledged. S.B. is grateful to Prof. Ira Weinstock assistance with the GC and UV experiments.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/8/25
Y1 - 2017/8/25
N2 - Carbon dots (C-dots) prepared through heating of aminoguanidine and citric acid enable bimodal (colorimetric and fluorescence) detection of nitric oxide (NO) in aqueous solutions. The C-dots retained the functional units of aminoguanidine, which upon reaction with NO produced surface residues responsible for the color and fluorescence transformations. Notably, the aminoguanidine/citric acid C-dots were noncytotoxic, making possible real-time and high sensitivity detection of NO in cellular environments. Using multiprong spectroscopic and chromatography analyses we deciphered the molecular mechanism accounting for the NO-induced structural and photophysical transformations of the C-dots, demonstrating for the first time N2 release and azo dye formation upon the C-dots' surface.
AB - Carbon dots (C-dots) prepared through heating of aminoguanidine and citric acid enable bimodal (colorimetric and fluorescence) detection of nitric oxide (NO) in aqueous solutions. The C-dots retained the functional units of aminoguanidine, which upon reaction with NO produced surface residues responsible for the color and fluorescence transformations. Notably, the aminoguanidine/citric acid C-dots were noncytotoxic, making possible real-time and high sensitivity detection of NO in cellular environments. Using multiprong spectroscopic and chromatography analyses we deciphered the molecular mechanism accounting for the NO-induced structural and photophysical transformations of the C-dots, demonstrating for the first time N2 release and azo dye formation upon the C-dots' surface.
KW - azo dye formation
KW - carbon dots
KW - fluorescence quenching
KW - nitric oxide detection
KW - polymerization of C-dots
UR - http://www.scopus.com/inward/record.url?scp=85028319283&partnerID=8YFLogxK
U2 - 10.1021/acssensors.7b00356
DO - 10.1021/acssensors.7b00356
M3 - Article
C2 - 28770991
AN - SCOPUS:85028319283
VL - 2
SP - 1215
EP - 1224
JO - ACS Sensors
JF - ACS Sensors
SN - 2379-3694
IS - 8
ER -