Nitrogen and oxygen isotopic signatures of subsurface nitrate seaward of the Florida Keys reef tract

James J. Leichter, Adina Paytan, Scott Wankel, Katharine Hanson, Steven Miller, Mark A. Altabet

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

Hydrographic and nutrient structure and the nitrogen and oxygen isotopic compositions (δ15N and δ18O) of the nitrate pool seaward of the Florida Keys reef tract were measured in Summer 2003. Nitrate and soluble reactive phosphate concentrations were tightly associated with depth and temperature and were close to 0 in surface waters, increased to 2-10 μmol L-1 and 0.1-1.0 μmol L-1, respectively, at intermediate depths associated with the thermocline and 15-25 μmol L -1 and 1.0-1.5 μmol L-1, respectively, in deeper water. Ammonium concentrations were low (0-0.6 μmol L-1) at all depths. Dissolved organic carbon concentrations ranged from 70-120 μmol L -1 near the surface to 40-60 μmol L-1 below the thermocline. Mean nitrate (δ15N was 4.78‰, (±0.05) overall and varied from 4.24‰, (±0.07) within the chlorophyll maximum layer at 50-70 m depth to 5.26‰ (±0.05) below the thermocline at 150-242 m depth. Mean δ18O mean was 3.26‰ (±0.08) and varied from 4.89‰ (±0.14) within the chlorophyll maximum layer to 3.02‰ (±0.07) below the thermocline. These observations suggest an oceanic, deep-water nitrate source below the thermocline, a mix of sources from upwelling and N2 fixation, and the effects of fractionation associated with uptake by primary producers within the thermocline. The water column nitrate isotopic data support the general hypothesis that primary producers on the Florida Keys reef tract directly utilize the transiently available subsurface nitrate pool.

Original languageEnglish
Pages (from-to)1258-1267
Number of pages10
JournalLimnology and Oceanography
Volume52
Issue number3
DOIs
StatePublished - 1 Jan 2007
Externally publishedYes

ASJC Scopus subject areas

  • Oceanography
  • Aquatic Science

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