Non-interferometric 3D fluorescence imaging for bio-applications

Osamu Matoba, Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Yosuke Tamada, Yasuhiro Awatsuji, Enrique Tajahuerce

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Non-interferometric three-dimensional (3D) fluorescence imaging techniques for bio-applications are presented. For bioapplications, the efficient illumination is very important to avoid the damage of living cells. We have proposed a method to obtain the complex amplitude of the fluorescence light wave with partially coherence by using the transport of intensity equation (TIE). We will show some experimental results of the reconstructed fluorescence distributions of fluorescence beads and living plant cells by TIE.

Original languageEnglish
Title of host publicationThree-Dimensional Imaging, Visualization, and Display 2020
EditorsBahram Javidi, Manuel Martinez-Corral, Osamu Matoba, Adrian Stern
ISBN (Electronic)9781510635814
StatePublished - 1 Jan 2020
Externally publishedYes
EventThree-Dimensional Imaging, Visualization, and Display 2020 - None, United States
Duration: 27 Apr 20208 May 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceThree-Dimensional Imaging, Visualization, and Display 2020
Country/TerritoryUnited States


  • 3D fluorescence imaging
  • Transport of intensity equation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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