Non-interferometric 3D fluorescence imaging for bio-applications

Osamu Matoba, Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Yosuke Tamada, Yasuhiro Awatsuji, Enrique Tajahuerce

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Non-interferometric three-dimensional (3D) fluorescence imaging techniques for bio-applications are presented. For bioapplications, the efficient illumination is very important to avoid the damage of living cells. We have proposed a method to obtain the complex amplitude of the fluorescence light wave with partially coherence by using the transport of intensity equation (TIE). We will show some experimental results of the reconstructed fluorescence distributions of fluorescence beads and living plant cells by TIE.

Original languageEnglish
Title of host publicationThree-Dimensional Imaging, Visualization, and Display 2020
EditorsBahram Javidi, Manuel Martinez-Corral, Osamu Matoba, Adrian Stern
PublisherSPIE
ISBN (Electronic)9781510635814
DOIs
StatePublished - 1 Jan 2020
Externally publishedYes
EventThree-Dimensional Imaging, Visualization, and Display 2020 - None, United States
Duration: 27 Apr 20208 May 2020

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11402
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceThree-Dimensional Imaging, Visualization, and Display 2020
Country/TerritoryUnited States
CityNone
Period27/04/208/05/20

Keywords

  • 3D fluorescence imaging
  • Transport of intensity equation

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