Non-interferometric 3D fluorescence imaging for bio-applications

Osamu Matoba; Sudheesh K. Rajput; Manoj Kumar; Xiangyu Quan; Yosuke Tamada; Yasuhiro Awatsuji; Enrique Tajahuerce

doi:10.1117/12.2559794

Non-interferometric 3D fluorescence imaging for bio-applications

Osamu Matoba, Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Yosuke Tamada, Yasuhiro Awatsuji, Enrique Tajahuerce

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Non-interferometric three-dimensional (3D) fluorescence imaging techniques for bio-applications are presented. For bioapplications, the efficient illumination is very important to avoid the damage of living cells. We have proposed a method to obtain the complex amplitude of the fluorescence light wave with partially coherence by using the transport of intensity equation (TIE). We will show some experimental results of the reconstructed fluorescence distributions of fluorescence beads and living plant cells by TIE.

Original language	English
Title of host publication	Three-Dimensional Imaging, Visualization, and Display 2020
Editors	Bahram Javidi, Manuel Martinez-Corral, Osamu Matoba, Adrian Stern
Publisher	SPIE
ISBN (Electronic)	9781510635814
DOIs	https://doi.org/10.1117/12.2559794
State	Published - 1 Jan 2020
Externally published	Yes
Event	Three-Dimensional Imaging, Visualization, and Display 2020 - None, United States Duration: 27 Apr 2020 → 8 May 2020

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	11402
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Three-Dimensional Imaging, Visualization, and Display 2020
Country/Territory	United States
City	None
Period	27/04/20 → 8/05/20

Keywords

3D fluorescence imaging
Transport of intensity equation

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.2559794

Cite this

Matoba, O., Rajput, S. K., Kumar, M., Quan, X., Tamada, Y., Awatsuji, Y., & Tajahuerce, E. (2020). Non-interferometric 3D fluorescence imaging for bio-applications. In B. Javidi, M. Martinez-Corral, O. Matoba, & A. Stern (Eds.), Three-Dimensional Imaging, Visualization, and Display 2020 Article 114020C (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11402). SPIE. https://doi.org/10.1117/12.2559794

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abstract = "Non-interferometric three-dimensional (3D) fluorescence imaging techniques for bio-applications are presented. For bioapplications, the efficient illumination is very important to avoid the damage of living cells. We have proposed a method to obtain the complex amplitude of the fluorescence light wave with partially coherence by using the transport of intensity equation (TIE). We will show some experimental results of the reconstructed fluorescence distributions of fluorescence beads and living plant cells by TIE.",

keywords = "3D fluorescence imaging, Transport of intensity equation",

author = "Osamu Matoba and Rajput, {Sudheesh K.} and Manoj Kumar and Xiangyu Quan and Yosuke Tamada and Yasuhiro Awatsuji and Enrique Tajahuerce",

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Matoba, O, Rajput, SK, Kumar, M, Quan, X, Tamada, Y, Awatsuji, Y & Tajahuerce, E 2020, Non-interferometric 3D fluorescence imaging for bio-applications. in B Javidi, M Martinez-Corral, O Matoba & A Stern (eds), Three-Dimensional Imaging, Visualization, and Display 2020., 114020C, Proceedings of SPIE - The International Society for Optical Engineering, vol. 11402, SPIE, Three-Dimensional Imaging, Visualization, and Display 2020, None, United States, 27/04/20. https://doi.org/10.1117/12.2559794

Non-interferometric 3D fluorescence imaging for bio-applications. / Matoba, Osamu; Rajput, Sudheesh K.; Kumar, Manoj et al.
Three-Dimensional Imaging, Visualization, and Display 2020. ed. / Bahram Javidi; Manuel Martinez-Corral; Osamu Matoba; Adrian Stern. SPIE, 2020. 114020C (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11402).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Rajput, Sudheesh K.

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AU - Awatsuji, Yasuhiro

AU - Tajahuerce, Enrique

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AB - Non-interferometric three-dimensional (3D) fluorescence imaging techniques for bio-applications are presented. For bioapplications, the efficient illumination is very important to avoid the damage of living cells. We have proposed a method to obtain the complex amplitude of the fluorescence light wave with partially coherence by using the transport of intensity equation (TIE). We will show some experimental results of the reconstructed fluorescence distributions of fluorescence beads and living plant cells by TIE.

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A2 - Javidi, Bahram

A2 - Martinez-Corral, Manuel

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ER -

Matoba O, Rajput SK, Kumar M, Quan X, Tamada Y, Awatsuji Y et al. Non-interferometric 3D fluorescence imaging for bio-applications. In Javidi B, Martinez-Corral M, Matoba O, Stern A, editors, Three-Dimensional Imaging, Visualization, and Display 2020. SPIE. 2020. 114020C. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2559794

Non-interferometric 3D fluorescence imaging for bio-applications

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