TY - JOUR
T1 - Non-photochemical quenching in the cells of the carotenogenic chlorophyte Haematococcus lacustris under favorable conditions and under stress
AU - Chekanov, K.
AU - Schastnaya, E.
AU - Neverov, K.
AU - Leu, S.
AU - Boussiba, S.
AU - Zarka, A.
AU - Solovchenko, A.
N1 - Funding Information:
A part of the work was supported by Council for grants of the President of the Russian Federation. The publication was prepared with the support of the «RUDN University Program 5-100». The research was conducted within the framework of the governmental Competitiveness Enhancement Program at the NRNU MEPhI and supported within the framework of a subsidy granted to the NRNU MEPhI by the Government of Russia for the implementation of the Global Competitiveness Program. We are thankful to Olivier Vallon and Nicolas Tourasse from the Institut de Biologie Physico-Chimique, University Pierre et Marie Curie (CNRS), France, for their dedicated work on the assembly and analysis of the H. lacustris transcriptome. Invaluable support of Dr. Olga Karpova, Dr. Elizaveta Vinogradova, and Dr. Sergey Gordeev (all from Lomonosov MSU) is appreciated.
Funding Information:
A part of the work was supported by Council for grants of the President of the Russian Federation. The publication was prepared with the support of the ?RUDN University Program 5-100?. The research was conducted within the framework of the governmental Competitiveness Enhancement Program at the NRNU MEPhI and supported within the framework of a subsidy granted to the NRNU MEPhI by the Government of Russia for the implementation of the Global Competitiveness Program. We are thankful to Olivier Vallon and Nicolas Tourasse from the Institut de Biologie Physico-Chimique, University Pierre et Marie Curie (CNRS), France, for their dedicated work on the assembly and analysis of the H. lacustris transcriptome. Invaluable support of Dr. Olga Karpova, Dr. Elizaveta Vinogradova, and Dr. Sergey Gordeev (all from Lomonosov MSU) is appreciated.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - The microalga Haematococcus lacustris (formerly H. pluvialis) is the richest source of the valuable pigment astaxanthin, accumulated in red aplanospores (haematocysts). In this work, we report on the photoprotective mechanisms in H. lacustris, conveying this microalga its ability to cope with a wide range of adverse conditions, with special emphasis put on non-photochemical quenching (NPQ) of the excited chlorophyll states. We studied the changes in the primary photochemistry of the photosystems (PS) as a function of irradiance and the physiological state. We leveraged the transcriptomic data to gain a deeper insight into possible NPQ mechanisms in this microalga. Peculiar to H. lacustris is a bi-phasic pattern of changes in photoprotection during haematocyst formation. The first phase coincides with a transient rise of photosynthetic activity. Based on transcriptomic data, high NPQ level in the first phase is maintained predominantly by the expression of PsbS and LhcsR proteins. Then, (in mature haematocysts), stress tolerance is achieved by optical shielding by astaxanthin and dramatic reduction of photosynthetic apparatus. In contrast to many microalgae, shielding plays an important role in H. lacistris haematocysts, whereas regulated NPQ is suppressed. Astaxanthin is decoupled from the PS, hence the light energy is not transferred to reaction centers and dissipates as heat. It allows to retain a higher photochemical yield in haematocysts comparing to vegetative cells. The ability of H. lacustris to substitute the “classical” active photoprotective mechanisms such as NPQ with optic shielding and general metabolism quiescence makes this organism a useful model to reveal photoprotection mechanisms.
AB - The microalga Haematococcus lacustris (formerly H. pluvialis) is the richest source of the valuable pigment astaxanthin, accumulated in red aplanospores (haematocysts). In this work, we report on the photoprotective mechanisms in H. lacustris, conveying this microalga its ability to cope with a wide range of adverse conditions, with special emphasis put on non-photochemical quenching (NPQ) of the excited chlorophyll states. We studied the changes in the primary photochemistry of the photosystems (PS) as a function of irradiance and the physiological state. We leveraged the transcriptomic data to gain a deeper insight into possible NPQ mechanisms in this microalga. Peculiar to H. lacustris is a bi-phasic pattern of changes in photoprotection during haematocyst formation. The first phase coincides with a transient rise of photosynthetic activity. Based on transcriptomic data, high NPQ level in the first phase is maintained predominantly by the expression of PsbS and LhcsR proteins. Then, (in mature haematocysts), stress tolerance is achieved by optical shielding by astaxanthin and dramatic reduction of photosynthetic apparatus. In contrast to many microalgae, shielding plays an important role in H. lacistris haematocysts, whereas regulated NPQ is suppressed. Astaxanthin is decoupled from the PS, hence the light energy is not transferred to reaction centers and dissipates as heat. It allows to retain a higher photochemical yield in haematocysts comparing to vegetative cells. The ability of H. lacustris to substitute the “classical” active photoprotective mechanisms such as NPQ with optic shielding and general metabolism quiescence makes this organism a useful model to reveal photoprotection mechanisms.
KW - Haematococcus lacustris
KW - LhcsR
KW - Non-photochemical quenching mechanisms
KW - Optical shielding
KW - Photoprotection
KW - PsbS
KW - Stress
UR - http://www.scopus.com/inward/record.url?scp=85067063278&partnerID=8YFLogxK
U2 - 10.1016/j.bbagen.2019.05.002
DO - 10.1016/j.bbagen.2019.05.002
M3 - Article
AN - SCOPUS:85067063278
SN - 0304-4165
VL - 1863
SP - 1429
EP - 1442
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 10
ER -