Non-scanning motionless fluorescence three-dimensional holographic microscopy

Joseph Rosen, Gary Brooker

Research output: Contribution to journalArticlepeer-review

411 Scopus citations

Abstract

Holography is an attractive imaging technique as it offers the ability to view a complete three-dimensional volume from one image. However, holography is not widely applied to the field of three-dimensional fluorescence microscopic imaging, because fluorescence is incoherent and creating holograms requires a coherent interferometer system. Although scanning one beam of an interferometer pattern across the rear aperture of an objective to excite fluorescence in a specimen overcomes the coherence limitation, the mechanical scanning is complicated, which makes the image capturing slow, and the process is limited to low-numerical-aperture objectives. Here we present the first demonstration of a motionless microscopy system (FINCHSCOPE) based on Fresnel incoherent correlation holography, and its use in recording high-resolution three-dimensional fluorescent images of biological specimens. By using high-numerical-aperture objectives, a spatial light modulator, a CCD camera and some simple filters, FINCHSCOPE enables the acquisition of three-dimensional microscopic images without the need for scanning.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalNature Photonics
Volume2
Issue number3
DOIs
StatePublished - 1 Mar 2008

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Fingerprint

Dive into the research topics of 'Non-scanning motionless fluorescence three-dimensional holographic microscopy'. Together they form a unique fingerprint.

Cite this