Nontransformed, GM-CSF-dependent macrophage lines are a unique model to study tissue macrophage functions

György Fejer, Mareike Dorothee Wegner, Ildiko Györy, Idan Cohen, Peggy Engelhard, Elena Voronov, Thomas Manke, Zsolt Ruzsics, Lars Dölken, Olivia Prazeres Da Costa, Nora Branzk, Michael Huber, Antje Prasse, Robert Schneider, Ron N. Apte, Chris Galanos, Marina A. Freudenberg

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Macrophages are diverse cell types in the first line of antimicrobial defense. Only a limited number of primary mouse models exist to study their function. Bone marrow-derived, macrophage-CSF-induced cells with a limited life span are the most common source. We report here a simple method yielding self-renewing, nontransformed, GM-CSF/signal transducer and activator of transcription 5-dependent macrophages (Max Planck Institute cells) from mouse fetal liver, which reflect the innate immune characteristics of alveolar macrophages. Max Planck Institute cells are exquisitely sensitive to selected microbial agents, including bacterial LPS, lipopeptide, Mycobacterium tuberculosis, cord factor, and adenovirus and mount highly proinflammatory but no anti-inflammatory IL-10 responses. They show a unique pattern of innate responses not yet observed in other mononuclear phagocytes. This includes differential LPS sensing and an unprecedented regulation of IL-1α production upon LPS exposure, which likely plays a key role in lung inflammation in vivo. In conclusion, Max Planck Institute cells offer an useful tool to study macrophage biology and for biomedical science.

Original languageEnglish
Pages (from-to)E2191-E2198
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number24
DOIs
StatePublished - 11 Jun 2013

Keywords

  • Innate immunity
  • LPS recognition

ASJC Scopus subject areas

  • General

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