Novel application for ion mobility spectrometry: Diagnosing vaginal infections through measurement of biogenic amines

Zeev Karpas, Walter Chaim, Rachel Gdalevsky, Boris Tilman, Avi Lorber

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

A method for diagnosis of bacterial vaginosis (BV) and other vaginal infections, based on measurement of biogenic amines present in a sample of vaginal fluid by ion mobility spectrometry (IMS) was developed. Sample introduction is through a two step procedure: addition of alkaline solution to release the volatile amines followed by heating and acid addition for emanation of the semi-volatile amines. Addition of n-nonylamine vapors to the carrier gas stream helps control the ionization processes and enhances the selective response to amines, even in the complex environment of biological matrices. A software package was developed for acquisition, storage and processing of the mobility spectra and for providing a diagnosis based on a table of rules. We report the results from testing of 210 samples of vaginal discharge fluid that were diagnosed by a gynecologist according to the widely used reference method (Amsel test) and by the new IMS method. The new method is rapid (less than 2min per sample), has a high sensitivity (few False Negatives) and specificity (few False Positives) with an accuracy of >95% for BV. The use of this method can reduce the incidence of misdiagnosis, particularly when trichomoniasis is confused with bacterial vaginosis.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalAnalytica Chimica Acta
Volume474
Issue number1-2
DOIs
StatePublished - 9 Dec 2002
Externally publishedYes

Keywords

  • Bacterial vaginosis
  • Biogenic amines
  • Ion mobility spectrometry (IMS)
  • Trichomoniasis
  • Vaginitis

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