Novel cationic vesicle platform derived from vernonia oil for efficient delivery of DNA through plant cuticle membranes

Zeev Wiesman, Naomi Ben Dom, Efrat Sharvit, Sarina Grinberg, Charles Linder, Eli Heldman, Michele Zaccai

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Novel cationic amphiphilic compounds were prepared from vernonia oil, a natural epoxidized triglyceride, and studied with respect to vesicle formation, encapsulation of biomaterials such as DNA, and their physical stability and transport through isolated plant cuticle membranes. The amphiphiles studied were a single-headed compound III (a quaternary ammonium head group with two alkyl chains) and a triple-headed compound IV, which is essentially three molecules of compound III bound together through a glycerol moiety. Vesicles of the two amphiphiles, prepared by sonication in water and solutions of uranyl acetate or the herbicide 2,4-D (2,4-dichloropenoxy acetic acid), were examined by TEM, SEM, AFM, and confocal laser systems and had a spherical shape which encapsulated the solutes with diameters between 40 and 110 nm. Vesicles from amphiphile IV could be made large enough to encapsulate a condensed 5.2 kb DNA plasmid (pJD328). Vesicles of amphiphile IV were also shown to pass intact across isolated plant cuticle membranes and the rate of delivery of encapsulated radio-labeled 2,4-D through isolated plant cuticle membranes obtained with these vesicles was clearly greater in comparison to liposomes prepared from dipalmitopyl phosphatidylcholine (DPPC) and the control, nonencapsulated 2,4-D. Vesicles from amphiphiles III and IV were found to be more stable than those of liposomes from DPPC. The data indicate the potential of vesicles prepared from the novel amphiphile IV to be a relatively efficient nano-scale delivery system to transport DNA and other bioactive agents through plant biological barriers. This scientific approach may open the way for further development of efficient in vivo plant transformation systems.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalJournal of Biotechnology
Volume130
Issue number1
DOIs
StatePublished - 31 May 2007

Keywords

  • Cuticle membrane
  • Dipalmitopyl phosphatidylcholine
  • Epoxy fatty acid
  • Nano-vesicles
  • Uranyl acetate
  • Vernonia oil

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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