TY - JOUR
T1 - Novel CRISPR/Cas technology in the realm of algal bloom biomonitoring
T2 - Recent trends and future perspectives
AU - Pal, Pracheta
AU - Anand, Uttpal
AU - Saha, Suchismita Chatterjee
AU - Sundaramurthy, Suresh
AU - Okeke, Emmanuel Sunday
AU - Kumar, Manoj
AU - Radha,
AU - Bontempi, Elza
AU - Albertini, Emidio
AU - Dey, Abhijit
AU - Di Maria, Francesco
N1 - Funding Information:
This work is not supported by any funding agency.
Publisher Copyright:
© 2023
PY - 2023/8/15
Y1 - 2023/8/15
N2 - In conjunction with global climate change, progressive ocean warming, and acclivity in pollution and anthropogenic eutrophication, the incidence of harmful algal blooms (HABs) and cyanobacterial harmful algal blooms (CHABs) continue to expand in distribution, frequency, and magnitude. Algal bloom-related toxins have been implicated in human health disorders and ecological dysfunction and are detrimental to the national and global economy. Biomonitoring programs based on traditional monitoring protocols were characterised by some limitations that can be efficiently overdone using the CRISPR/Cas technology. In the present review, the potential and challenges of exploiting the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas technology for early detection of HABs and CHABs-associated toxigenic species were analysed. Based on more than 30 scientific papers, the main results indicate the great potential of CRISPR/Cas technology for this issue, even if the high sensitivity detected for the Cas12 and Cas13 platforms represents a possible interference risk.
AB - In conjunction with global climate change, progressive ocean warming, and acclivity in pollution and anthropogenic eutrophication, the incidence of harmful algal blooms (HABs) and cyanobacterial harmful algal blooms (CHABs) continue to expand in distribution, frequency, and magnitude. Algal bloom-related toxins have been implicated in human health disorders and ecological dysfunction and are detrimental to the national and global economy. Biomonitoring programs based on traditional monitoring protocols were characterised by some limitations that can be efficiently overdone using the CRISPR/Cas technology. In the present review, the potential and challenges of exploiting the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas technology for early detection of HABs and CHABs-associated toxigenic species were analysed. Based on more than 30 scientific papers, the main results indicate the great potential of CRISPR/Cas technology for this issue, even if the high sensitivity detected for the Cas12 and Cas13 platforms represents a possible interference risk.
KW - Biomonitoring and biosensing
KW - CRISPR/Cas technology
KW - HAB-Related toxins
KW - Harmful algal blooms (HABs)
KW - Nucleic acid detection
KW - Toxin-producing genera
UR - http://www.scopus.com/inward/record.url?scp=85160097031&partnerID=8YFLogxK
U2 - 10.1016/j.envres.2023.115989
DO - 10.1016/j.envres.2023.115989
M3 - Article
C2 - 37119838
AN - SCOPUS:85160097031
SN - 0013-9351
VL - 231
JO - Environmental Research
JF - Environmental Research
M1 - 115989
ER -
