Novel cry-type genes detected by extended PCR screening from field-collected strains of Bacillus thuringiensis

E. Ben-Dov, E. Dahan, A. Zaritsky, Z. Barak, R. Sinai, R. Manasherob, A. Khameraev, A. Troyetskaya, A. Dubitsky, N. Berezina, Y. Margalith

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A rapid method for identification of Bacillus thuringiensis strains was established by using extended multiplex PCR (Ben Dov et al., 1997). Using this method we found seven distinct cry-type profiles and recorded their distribution in Israel, Uzbekistan and Kazakhstan. Three new genes with potential insecticidal activity were identified. One of them seems to be a naturally occurring recombinant, apparently composed of cryI- and cryIV-type genes. The other two (apparently homologous to cryIIIF and cryIIIG) seem to include various combinations with either cryI or cryIV.

Original languageEnglish
Pages (from-to)163-169
Number of pages7
JournalIsrael Journal of Entomology
Volume32
StatePublished - 1 Dec 1998

Keywords

  • Bacillus thuringiensis
  • Biological control of pests
  • PCR screening
  • cry genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (all)

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