Novel cry-type genes detected by extended PCR screening from field-collected strains of Bacillus thuringiensis

E. Ben-Dov; E. Dahan; A. Zaritsky; Z. Barak; R. Sinai; R. Manasherob; A. Khameraev; A. Troyetskaya; A. Dubitsky; N. Berezina; Y. Margalith

Novel cry-type genes detected by extended PCR screening from field-collected strains of Bacillus thuringiensis

E. Ben-Dov, E. Dahan, A. Zaritsky, Z. Barak, R. Sinai, R. Manasherob, A. Khameraev, A. Troyetskaya, A. Dubitsky, N. Berezina, Y. Margalith

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A rapid method for identification of Bacillus thuringiensis strains was established by using extended multiplex PCR (Ben Dov et al., 1997). Using this method we found seven distinct cry-type profiles and recorded their distribution in Israel, Uzbekistan and Kazakhstan. Three new genes with potential insecticidal activity were identified. One of them seems to be a naturally occurring recombinant, apparently composed of cryI- and cryIV-type genes. The other two (apparently homologous to cryIIIF and cryIIIG) seem to include various combinations with either cryI or cryIV.

Original language	English
Pages (from-to)	163-169
Number of pages	7
Journal	Israel Journal of Entomology
Volume	32
State	Published - 1 Dec 1998

Keywords

Bacillus thuringiensis
Biological control of pests
PCR screening
cry genes

ASJC Scopus subject areas

General Agricultural and Biological Sciences

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Novel cry-type genes detected by extended PCR screening from field-collected strains of Bacillus thuringiensis",

abstract = "A rapid method for identification of Bacillus thuringiensis strains was established by using extended multiplex PCR (Ben Dov et al., 1997). Using this method we found seven distinct cry-type profiles and recorded their distribution in Israel, Uzbekistan and Kazakhstan. Three new genes with potential insecticidal activity were identified. One of them seems to be a naturally occurring recombinant, apparently composed of cryI- and cryIV-type genes. The other two (apparently homologous to cryIIIF and cryIIIG) seem to include various combinations with either cryI or cryIV.",

keywords = "Bacillus thuringiensis, Biological control of pests, PCR screening, cry genes",

author = "E. Ben-Dov and E. Dahan and A. Zaritsky and Z. Barak and R. Sinai and R. Manasherob and A. Khameraev and A. Troyetskaya and A. Dubitsky and N. Berezina and Y. Margalith",

year = "1998",

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language = "English",

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pages = "163--169",

journal = "Israel Journal of Entomology",

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T1 - Novel cry-type genes detected by extended PCR screening from field-collected strains of Bacillus thuringiensis

AU - Ben-Dov, E.

AU - Dahan, E.

AU - Zaritsky, A.

AU - Barak, Z.

AU - Sinai, R.

AU - Manasherob, R.

AU - Khameraev, A.

AU - Troyetskaya, A.

AU - Dubitsky, A.

AU - Berezina, N.

AU - Margalith, Y.

PY - 1998/12/1

Y1 - 1998/12/1

N2 - A rapid method for identification of Bacillus thuringiensis strains was established by using extended multiplex PCR (Ben Dov et al., 1997). Using this method we found seven distinct cry-type profiles and recorded their distribution in Israel, Uzbekistan and Kazakhstan. Three new genes with potential insecticidal activity were identified. One of them seems to be a naturally occurring recombinant, apparently composed of cryI- and cryIV-type genes. The other two (apparently homologous to cryIIIF and cryIIIG) seem to include various combinations with either cryI or cryIV.

AB - A rapid method for identification of Bacillus thuringiensis strains was established by using extended multiplex PCR (Ben Dov et al., 1997). Using this method we found seven distinct cry-type profiles and recorded their distribution in Israel, Uzbekistan and Kazakhstan. Three new genes with potential insecticidal activity were identified. One of them seems to be a naturally occurring recombinant, apparently composed of cryI- and cryIV-type genes. The other two (apparently homologous to cryIIIF and cryIIIG) seem to include various combinations with either cryI or cryIV.

KW - Bacillus thuringiensis

KW - Biological control of pests

KW - PCR screening

KW - cry genes

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M3 - Article

AN - SCOPUS:26944465798

SN - 0075-1243

VL - 32

SP - 163

EP - 169

JO - Israel Journal of Entomology

JF - Israel Journal of Entomology

ER -

Novel cry-type genes detected by extended PCR screening from field-collected strains of Bacillus thuringiensis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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