A rapid method for identification of Bacillus thuringiensis strains was established by using extended multiplex PCR (Ben Dov et al., 1997). Using this method we found seven distinct cry-type profiles and recorded their distribution in Israel, Uzbekistan and Kazakhstan. Three new genes with potential insecticidal activity were identified. One of them seems to be a naturally occurring recombinant, apparently composed of cryI- and cryIV-type genes. The other two (apparently homologous to cryIIIF and cryIIIG) seem to include various combinations with either cryI or cryIV.
|Number of pages||7|
|Journal||Israel Journal of Entomology|
|State||Published - 1 Dec 1998|
- Bacillus thuringiensis
- Biological control of pests
- PCR screening
- cry genes