Novel fluorescence-photochrome labeling method in the study of biomembrane dynamics

G. I. Likhtenshtein; Rizak Bishara; V. Papper; B. Uzan; I. Fishov; D. Gill; A. H. Parola

doi:10.1016/S0165-022X(96)00022-X

Novel fluorescence-photochrome labeling method in the study of biomembrane dynamics

G. I. Likhtenshtein, Rizak Bishara, V. Papper, B. Uzan, I. Fishov, D. Gill, A. H. Parola

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

A novel photochrome-fluorescence method (PFLM) based on monitoring fluorescence parameters and kinetics of photochrome photoisomerization of para-substituted stilbenes (PSS) has been proposed. It was shown that PSS exhibits fluorescence characteristics which are similar to ones of typical membrane fluorescence probes such as diphenylhexatriene (DPH). A study of kinetics of PSS trans-cis and cis-trans photoisomerization makes it possible to estimate, under certain conditions, the rotational correlation time of the stilbene fragments in the excited state of PSS for the fixed angle 180°. In viscous media this process is a rate-determining stage. Taken together, the both techniques, fluorescence and photochrome, make it possible to establish a detailed mechanism and measure quantitative parameters of stilbene probe (PSS) mobility in a membrane. The PFLM was applied to the study of E. coli membrane dynamics.

Original language	English
Pages (from-to)	117-133
Number of pages	17
Journal	Journal of Biochemical and Biophysical Methods
Volume	33
Issue number	2
DOIs	https://doi.org/10.1016/S0165-022X(96)00022-X
State	Published - 15 Nov 1996

Keywords

Biomembrane
Fluorescence polarization
Microviscosity
Photochrome label
Stilbene
Trans-cis isomerization

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.1016/S0165-022X(96)00022-X

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title = "Novel fluorescence-photochrome labeling method in the study of biomembrane dynamics",

abstract = "A novel photochrome-fluorescence method (PFLM) based on monitoring fluorescence parameters and kinetics of photochrome photoisomerization of para-substituted stilbenes (PSS) has been proposed. It was shown that PSS exhibits fluorescence characteristics which are similar to ones of typical membrane fluorescence probes such as diphenylhexatriene (DPH). A study of kinetics of PSS trans-cis and cis-trans photoisomerization makes it possible to estimate, under certain conditions, the rotational correlation time of the stilbene fragments in the excited state of PSS for the fixed angle 180°. In viscous media this process is a rate-determining stage. Taken together, the both techniques, fluorescence and photochrome, make it possible to establish a detailed mechanism and measure quantitative parameters of stilbene probe (PSS) mobility in a membrane. The PFLM was applied to the study of E. coli membrane dynamics.",

keywords = "Biomembrane, Fluorescence polarization, Microviscosity, Photochrome label, Stilbene, Trans-cis isomerization",

author = "Likhtenshtein, {G. I.} and Rizak Bishara and V. Papper and B. Uzan and I. Fishov and D. Gill and Parola, {A. H.}",

year = "1996",

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AU - Likhtenshtein, G. I.

AU - Bishara, Rizak

AU - Papper, V.

AU - Uzan, B.

AU - Fishov, I.

AU - Gill, D.

AU - Parola, A. H.

PY - 1996/11/15

Y1 - 1996/11/15

N2 - A novel photochrome-fluorescence method (PFLM) based on monitoring fluorescence parameters and kinetics of photochrome photoisomerization of para-substituted stilbenes (PSS) has been proposed. It was shown that PSS exhibits fluorescence characteristics which are similar to ones of typical membrane fluorescence probes such as diphenylhexatriene (DPH). A study of kinetics of PSS trans-cis and cis-trans photoisomerization makes it possible to estimate, under certain conditions, the rotational correlation time of the stilbene fragments in the excited state of PSS for the fixed angle 180°. In viscous media this process is a rate-determining stage. Taken together, the both techniques, fluorescence and photochrome, make it possible to establish a detailed mechanism and measure quantitative parameters of stilbene probe (PSS) mobility in a membrane. The PFLM was applied to the study of E. coli membrane dynamics.

AB - A novel photochrome-fluorescence method (PFLM) based on monitoring fluorescence parameters and kinetics of photochrome photoisomerization of para-substituted stilbenes (PSS) has been proposed. It was shown that PSS exhibits fluorescence characteristics which are similar to ones of typical membrane fluorescence probes such as diphenylhexatriene (DPH). A study of kinetics of PSS trans-cis and cis-trans photoisomerization makes it possible to estimate, under certain conditions, the rotational correlation time of the stilbene fragments in the excited state of PSS for the fixed angle 180°. In viscous media this process is a rate-determining stage. Taken together, the both techniques, fluorescence and photochrome, make it possible to establish a detailed mechanism and measure quantitative parameters of stilbene probe (PSS) mobility in a membrane. The PFLM was applied to the study of E. coli membrane dynamics.

KW - Biomembrane

KW - Fluorescence polarization

KW - Microviscosity

KW - Photochrome label

KW - Stilbene

KW - Trans-cis isomerization

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M3 - Article

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SN - 0165-022X

VL - 33

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JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

IS - 2

ER -

Novel fluorescence-photochrome labeling method in the study of biomembrane dynamics

Abstract

Keywords

ASJC Scopus subject areas

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