Novel fluorescence-photochrome labeling method in the study of biomembrane dynamics

G. I. Likhtenshtein, Rizak Bishara, V. Papper, B. Uzan, I. Fishov, D. Gill, A. H. Parola

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

A novel photochrome-fluorescence method (PFLM) based on monitoring fluorescence parameters and kinetics of photochrome photoisomerization of para-substituted stilbenes (PSS) has been proposed. It was shown that PSS exhibits fluorescence characteristics which are similar to ones of typical membrane fluorescence probes such as diphenylhexatriene (DPH). A study of kinetics of PSS trans-cis and cis-trans photoisomerization makes it possible to estimate, under certain conditions, the rotational correlation time of the stilbene fragments in the excited state of PSS for the fixed angle 180°. In viscous media this process is a rate-determining stage. Taken together, the both techniques, fluorescence and photochrome, make it possible to establish a detailed mechanism and measure quantitative parameters of stilbene probe (PSS) mobility in a membrane. The PFLM was applied to the study of E. coli membrane dynamics.

Original languageEnglish
Pages (from-to)117-133
Number of pages17
JournalJournal of Biochemical and Biophysical Methods
Volume33
Issue number2
DOIs
StatePublished - 15 Nov 1996

Keywords

  • Biomembrane
  • Fluorescence polarization
  • Microviscosity
  • Photochrome label
  • Stilbene
  • Trans-cis isomerization

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