Novel fluorescent methods for biotechnological and biomedical sensoring: Assessing antioxidants, reactive radicals, NO dynamics, immunoassay, and biomembranes fluidity

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21 Scopus citations

Abstract

We proposed and developed a series of fluorescent methods for analysis and investigation of biological systems with a view of future biotechnological and biomedical applications. The methods we describe have been built upon several photochemical and photophysical phenomena including fluorescent quenching, photochrome photoisomerization, and energy transfer. Three new types of molecular probes have been developed and employed for such studies: (1) dual fluorophore-nitroxide compounds, (2) fluorescence-photochrome molecules, and (3) super molecules containing both fluorescence and fluorescent quenching segments. The fluorescent properties of the new probes were intensively exploited for several practical applications including a real-time analysis of antioxidants, nitric oxide, superoxide, reactive radicals, trinitrotoluene, and metal ions, investigation of molecular dynamics of biomembranes in a wide range characteristic times, detection of protein conformational transition, and characterization of surface system. Owning high sensitivity, simplicity, and availability of fluorescent techniques, these methods can be widely employed and are adaptable to fibrooptic sensoring. A general survey of the physical principles and application of the new fluorescent methods has been provided.

Original languageEnglish
Pages (from-to)135-155
Number of pages21
JournalApplied Biochemistry and Biotechnology
Volume152
Issue number1
DOIs
StatePublished - 1 Jan 2009

Keywords

  • Antibodies
  • Antioxidants
  • Biomembrane fluidity
  • Fluorephore-nitroxides
  • Fluorescence
  • Fluorescent quenching
  • Immunoassay
  • Nitric oxides
  • Photochrome isomerization
  • Singlet-singlet energy transfer
  • Stilbenes
  • Superoxide

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