Novel hybrid esterase-haloacid dehalogenase enzyme

Ana Beloqui; Julio Polaina; José María Vieites; Dolores Reyes-Duarte; Rodrigo Torres; Olga V. Golyshina; Tatyana N. Chernikova; Agnes Waliczek; Amir Aharoni; Michail M. Yakimov; Kenneth N. Timmis; Peter N. Golyshin; Manuel Ferrer

doi:10.1002/cbic.201000258

Novel hybrid esterase-haloacid dehalogenase enzyme

Ana Beloqui, Julio Polaina, José María Vieites, Dolores Reyes-Duarte, Rodrigo Torres, Olga V. Golyshina, Tatyana N. Chernikova, Agnes Waliczek, Amir Aharoni, Michail M. Yakimov, Kenneth N. Timmis, Peter N. Golyshin, Manuel Ferrer

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

(Figure Presented) Promiscuous proteins: The identification and characterization of a wild-type promiscuous hydrolase that efficiently catalyzes the hydrolysis of ester bonds and haloacids in a single protein has been reported. This study has demonstrated the utility of metagenomics to access novel catalytic activities.

Original language	English
Pages (from-to)	1975-1978
Number of pages	4
Journal	ChemBioChem
Volume	11
Issue number	14
DOIs	https://doi.org/10.1002/cbic.201000258
State	Published - 24 Sep 2010

Keywords

Dehalogenases
Esterases
Hydrolysis
Metagenomes
Protein design

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.201000258

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title = "Novel hybrid esterase-haloacid dehalogenase enzyme",

abstract = "(Figure Presented) Promiscuous proteins: The identification and characterization of a wild-type promiscuous hydrolase that efficiently catalyzes the hydrolysis of ester bonds and haloacids in a single protein has been reported. This study has demonstrated the utility of metagenomics to access novel catalytic activities.",

keywords = "Dehalogenases, Esterases, Hydrolysis, Metagenomes, Protein design",

author = "Ana Beloqui and Julio Polaina and Vieites, {Jos{\'e} Mar{\'i}a} and Dolores Reyes-Duarte and Rodrigo Torres and Golyshina, {Olga V.} and Chernikova, {Tatyana N.} and Agnes Waliczek and Amir Aharoni and Yakimov, {Michail M.} and Timmis, {Kenneth N.} and Golyshin, {Peter N.} and Manuel Ferrer",

year = "2010",

month = sep,

day = "24",

doi = "10.1002/cbic.201000258",

language = "English",

volume = "11",

pages = "1975--1978",

journal = "ChemBioChem",

issn = "1439-4227",

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T1 - Novel hybrid esterase-haloacid dehalogenase enzyme

AU - Beloqui, Ana

AU - Polaina, Julio

AU - Vieites, José María

AU - Reyes-Duarte, Dolores

AU - Torres, Rodrigo

AU - Golyshina, Olga V.

AU - Chernikova, Tatyana N.

AU - Waliczek, Agnes

AU - Aharoni, Amir

AU - Yakimov, Michail M.

AU - Timmis, Kenneth N.

AU - Golyshin, Peter N.

AU - Ferrer, Manuel

PY - 2010/9/24

Y1 - 2010/9/24

N2 - (Figure Presented) Promiscuous proteins: The identification and characterization of a wild-type promiscuous hydrolase that efficiently catalyzes the hydrolysis of ester bonds and haloacids in a single protein has been reported. This study has demonstrated the utility of metagenomics to access novel catalytic activities.

AB - (Figure Presented) Promiscuous proteins: The identification and characterization of a wild-type promiscuous hydrolase that efficiently catalyzes the hydrolysis of ester bonds and haloacids in a single protein has been reported. This study has demonstrated the utility of metagenomics to access novel catalytic activities.

KW - Dehalogenases

KW - Esterases

KW - Hydrolysis

KW - Metagenomes

KW - Protein design

UR - http://www.scopus.com/inward/record.url?scp=77957203926&partnerID=8YFLogxK

U2 - 10.1002/cbic.201000258

DO - 10.1002/cbic.201000258

M3 - Article

C2 - 20715265

AN - SCOPUS:77957203926

SN - 1439-4227

VL - 11

SP - 1975

EP - 1978

JO - ChemBioChem

JF - ChemBioChem

IS - 14

ER -

Novel hybrid esterase-haloacid dehalogenase enzyme

Abstract

Keywords

ASJC Scopus subject areas

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