Novel method for measuring polyuridylic acid binding to ribosomes

Moshe Smolarsky, Moshe Tal

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Nitrocellulose membranes, mildly pretreated with alkali, lose their ability to adsorb poly U, but retain their potentiality for binding ribosomes. According to this observation, a new method for measuring poly U binding to ribosomes is proposed. Results indicate that both the secondary structure of poly U and the chemical nature of the nitrocellulose filter influence the interaction of the polymer and the membrane.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume199
Issue number2
DOIs
StatePublished - 18 Feb 1970
Externally publishedYes

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