Novel methodology for the follow-up of acute lymphoblastic leukemia using FTIR microspectroscopy

J. Ramesh, J. Kapelushnik, J. Mordehai, A. Moser, M. Huleihel, V. Erukhimovitch, C. Levi, S. Mordechai

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

In this report, we present a novel spectroscopic method of follow-up during chemotherapy treatment for B- and T-cell childhood leukemia patients. We isolated peripheral lymphocytes from blood drawn from patients before and after the chemotherapy and collected Microscopic FTIR (FTIR-MC) spectra of the isolated lymphocytes. Our results showed that nucleic acids content decreased in both types of patients. Changes in phospholipids and proteins level could be observed. The overall effects of drugs administered to the patients can be understood at the molecular level using FTIR-MC and these results are expected to stimulate wider applications of spectroscopy in leukemia research.

Original languageEnglish
Pages (from-to)251-261
Number of pages11
JournalJournal of Biochemical and Biophysical Methods
Volume51
Issue number3
DOIs
StatePublished - 31 May 2002

Keywords

  • Acute lymphoblastic leukemia
  • Drugs
  • FTIR microspectroscopy
  • Follow-up
  • Lymphocytes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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