Novel non-covalent conjugate based on graphene oxide and alkylating agent from 1,3,5-triazine class

Vladimir V. Sharoyko; Olga V. Mikolaichuk; Olga S. Shemchuk; Abdelsattar O.E. Abdelhalim; Artem A. Potanin; Mikhail D. Luttsev; Daler R. Dadadzhanov; Tigran A. Vartanyan; Andrey V. Petrov; Anna Yu Shasherina; Igor V. Murin; Dmitrii N. Maistrenko; Oleg E. Molchanov; Konstantin N. Semenov

doi:10.1016/j.molliq.2023.121203

Novel non-covalent conjugate based on graphene oxide and alkylating agent from 1,3,5-triazine class

Vladimir V. Sharoyko, Olga V. Mikolaichuk, Olga S. Shemchuk, Abdelsattar O.E. Abdelhalim, Artem A. Potanin, Mikhail D. Luttsev, Daler R. Dadadzhanov, Tigran A. Vartanyan, Andrey V. Petrov, Anna Yu Shasherina, Igor V. Murin, Dmitrii N. Maistrenko, Oleg E. Molchanov, Konstantin N. Semenov

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

One of the ways to increase the specificity and biocompatibility of cytostatic drugs is the creation of conjugates of drugs with graphene oxide. Graphene and its oxidized form - graphene oxide (GO) - have become new promising materials in the field of nanomedicine due to its reactive and developed surface that can be subjected to covalent and non-covalent functionalization, which allows provide the immobilization of drugs. This article is devoted to a new method for the synthesis, identification and study of the biocompatibility of a non-covalent conjugate based on GO and an alkylating agent based on 1,3,5-triazine. The conjugates are hemocompatible (the degree of hemolysis does not exceed 5 % in the concentration range 1–200 mg∙l⁻¹), exhibit antioxidant activity in the model reaction with DPPH, Radachlorin (the photodegradation constant decreased by 1.7 times at the maximum concentration of GO-1 75 mg∙l⁻¹) and photoinduced hemolysis, has dose-dependent genotoxicity, and also exhibit cytotoxicity towards cell lines A549, PANC-1 and HeLa. The maximum cytotoxicity is shown in the HeLa cell line (IC₅₀ = 2.5 µM).

Original language	English
Article number	121203
Journal	Journal of Molecular Liquids
Volume	372
DOIs	https://doi.org/10.1016/j.molliq.2023.121203
State	Published - 15 Feb 2023
Externally published	Yes

Keywords

1,3,5-triazine
Antioxidant activity
Cytotoxicity
Genotoxicity
Graphene oxide
Hemocompatibility

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Condensed Matter Physics
Spectroscopy
Physical and Theoretical Chemistry
Materials Chemistry

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10.1016/j.molliq.2023.121203

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Sharoyko, V. V., Mikolaichuk, O. V., Shemchuk, O. S., Abdelhalim, A. O. E., Potanin, A. A., Luttsev, M. D., Dadadzhanov, D. R., Vartanyan, T. A., Petrov, A. V., Shasherina, A. Y., Murin, I. V., Maistrenko, D. N., Molchanov, O. E., & Semenov, K. N. (2023). Novel non-covalent conjugate based on graphene oxide and alkylating agent from 1,3,5-triazine class. Journal of Molecular Liquids, 372, Article 121203. https://doi.org/10.1016/j.molliq.2023.121203

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title = "Novel non-covalent conjugate based on graphene oxide and alkylating agent from 1,3,5-triazine class",

abstract = "One of the ways to increase the specificity and biocompatibility of cytostatic drugs is the creation of conjugates of drugs with graphene oxide. Graphene and its oxidized form - graphene oxide (GO) - have become new promising materials in the field of nanomedicine due to its reactive and developed surface that can be subjected to covalent and non-covalent functionalization, which allows provide the immobilization of drugs. This article is devoted to a new method for the synthesis, identification and study of the biocompatibility of a non-covalent conjugate based on GO and an alkylating agent based on 1,3,5-triazine. The conjugates are hemocompatible (the degree of hemolysis does not exceed 5 % in the concentration range 1–200 mg∙l−1), exhibit antioxidant activity in the model reaction with DPPH, Radachlorin (the photodegradation constant decreased by 1.7 times at the maximum concentration of GO-1 75 mg∙l−1) and photoinduced hemolysis, has dose-dependent genotoxicity, and also exhibit cytotoxicity towards cell lines A549, PANC-1 and HeLa. The maximum cytotoxicity is shown in the HeLa cell line (IC50 = 2.5 µM).",

keywords = "1,3,5-triazine, Antioxidant activity, Cytotoxicity, Genotoxicity, Graphene oxide, Hemocompatibility",

author = "Sharoyko, {Vladimir V.} and Mikolaichuk, {Olga V.} and Shemchuk, {Olga S.} and Abdelhalim, {Abdelsattar O.E.} and Potanin, {Artem A.} and Luttsev, {Mikhail D.} and Dadadzhanov, {Daler R.} and Vartanyan, {Tigran A.} and Petrov, {Andrey V.} and Shasherina, {Anna Yu} and Murin, {Igor V.} and Maistrenko, {Dmitrii N.} and Molchanov, {Oleg E.} and Semenov, {Konstantin N.}",

note = "Publisher Copyright: {\textcopyright} 2023",

year = "2023",

month = feb,

day = "15",

doi = "10.1016/j.molliq.2023.121203",

language = "English",

volume = "372",

journal = "Journal of Molecular Liquids",

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Sharoyko, VV, Mikolaichuk, OV, Shemchuk, OS, Abdelhalim, AOE, Potanin, AA, Luttsev, MD, Dadadzhanov, DR, Vartanyan, TA, Petrov, AV, Shasherina, AY, Murin, IV, Maistrenko, DN, Molchanov, OE & Semenov, KN 2023, 'Novel non-covalent conjugate based on graphene oxide and alkylating agent from 1,3,5-triazine class', Journal of Molecular Liquids, vol. 372, 121203. https://doi.org/10.1016/j.molliq.2023.121203

TY - JOUR

T1 - Novel non-covalent conjugate based on graphene oxide and alkylating agent from 1,3,5-triazine class

AU - Sharoyko, Vladimir V.

AU - Mikolaichuk, Olga V.

AU - Shemchuk, Olga S.

AU - Abdelhalim, Abdelsattar O.E.

AU - Potanin, Artem A.

AU - Luttsev, Mikhail D.

AU - Dadadzhanov, Daler R.

AU - Vartanyan, Tigran A.

AU - Petrov, Andrey V.

AU - Shasherina, Anna Yu

AU - Murin, Igor V.

AU - Maistrenko, Dmitrii N.

AU - Molchanov, Oleg E.

AU - Semenov, Konstantin N.

PY - 2023/2/15

Y1 - 2023/2/15

N2 - One of the ways to increase the specificity and biocompatibility of cytostatic drugs is the creation of conjugates of drugs with graphene oxide. Graphene and its oxidized form - graphene oxide (GO) - have become new promising materials in the field of nanomedicine due to its reactive and developed surface that can be subjected to covalent and non-covalent functionalization, which allows provide the immobilization of drugs. This article is devoted to a new method for the synthesis, identification and study of the biocompatibility of a non-covalent conjugate based on GO and an alkylating agent based on 1,3,5-triazine. The conjugates are hemocompatible (the degree of hemolysis does not exceed 5 % in the concentration range 1–200 mg∙l−1), exhibit antioxidant activity in the model reaction with DPPH, Radachlorin (the photodegradation constant decreased by 1.7 times at the maximum concentration of GO-1 75 mg∙l−1) and photoinduced hemolysis, has dose-dependent genotoxicity, and also exhibit cytotoxicity towards cell lines A549, PANC-1 and HeLa. The maximum cytotoxicity is shown in the HeLa cell line (IC50 = 2.5 µM).

AB - One of the ways to increase the specificity and biocompatibility of cytostatic drugs is the creation of conjugates of drugs with graphene oxide. Graphene and its oxidized form - graphene oxide (GO) - have become new promising materials in the field of nanomedicine due to its reactive and developed surface that can be subjected to covalent and non-covalent functionalization, which allows provide the immobilization of drugs. This article is devoted to a new method for the synthesis, identification and study of the biocompatibility of a non-covalent conjugate based on GO and an alkylating agent based on 1,3,5-triazine. The conjugates are hemocompatible (the degree of hemolysis does not exceed 5 % in the concentration range 1–200 mg∙l−1), exhibit antioxidant activity in the model reaction with DPPH, Radachlorin (the photodegradation constant decreased by 1.7 times at the maximum concentration of GO-1 75 mg∙l−1) and photoinduced hemolysis, has dose-dependent genotoxicity, and also exhibit cytotoxicity towards cell lines A549, PANC-1 and HeLa. The maximum cytotoxicity is shown in the HeLa cell line (IC50 = 2.5 µM).

KW - 1,3,5-triazine

KW - Antioxidant activity

KW - Cytotoxicity

KW - Genotoxicity

KW - Graphene oxide

KW - Hemocompatibility

UR - http://www.scopus.com/inward/record.url?scp=85146050986&partnerID=8YFLogxK

U2 - 10.1016/j.molliq.2023.121203

DO - 10.1016/j.molliq.2023.121203

M3 - Article

AN - SCOPUS:85146050986

SN - 0167-7322

VL - 372

JO - Journal of Molecular Liquids

JF - Journal of Molecular Liquids

M1 - 121203

ER -

Novel non-covalent conjugate based on graphene oxide and alkylating agent from 1,3,5-triazine class

Abstract

Keywords

ASJC Scopus subject areas

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