Novel spectral method for the study of viral carcinogenesis in vitro

Mahmoud Huleihel, Ahmad Salman, Vitaly Erukhimovitch, Jagannathan Ramesh, Ziad Hammody, Shaul Mordechai

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Fourier transform infrared (FTIR) spectroscopy is a unique technique for the laboratory diagnosis of cellular variations based on the characteristic molecular vibrational spectra of the cells. Microscopic FTIR was used to investigate spectral differences between normal and malignant fibroblasts transformed by retrovirus infection. A detailed analysis showed significant differences between cancerous and normal cells. The contents of vital cellular metabolites were significantly lower in the transformed cells than in the normal cells. In an attempt to identify the cellular components responsible for the observed spectral differences between normal and cancerous cells, we found significant differences between DNA of normal and cancerous cells.

Original languageEnglish
Pages (from-to)111-121
Number of pages11
JournalJournal of Biochemical and Biophysical Methods
Volume50
Issue number2-3
DOIs
StatePublished - 4 Jan 2002

Keywords

  • Cancer
  • Diagnosis
  • FTIR microscopy
  • IR spectroscopy
  • Malignant cells
  • NIH/3T3 cells
  • Retroviruses

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Fingerprint

Dive into the research topics of 'Novel spectral method for the study of viral carcinogenesis in vitro'. Together they form a unique fingerprint.

Cite this