OffRisk: A docker image for annotating CRISPR off-target sites in the human genome

Gil Ad Barkai, Tal Malul, Yossi Eliaz, Eran Eyal, Isana Veksler-Lublinsky

Research output: Contribution to journalArticlepeer-review

Abstract

The CRISPR-Cas9 system has been adapted to achieve targeted genome editing as well as transcriptional control by customizing 20-nt guide RNA (gRNA) molecules for desired regions in the target genome. Designing gRNAs must consider nonspecific and unintended binding, known as off-targets, since these may have potentially harmful effects. To assist in gRNA design, we have developed OffRisk. This Docker-based tool annotates off-target sites in the human genome and assigns them a potential risk label by incorporating functional and regulatory information at different molecular levels.

Original languageEnglish
Article numbervbad138
JournalBioinformatics Advances
Volume3
Issue number1
DOIs
StatePublished - 1 Jan 2023

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Structural Biology
  • Computer Science Applications

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