TY - JOUR
T1 - OffRisk
T2 - A docker image for annotating CRISPR off-target sites in the human genome
AU - Barkai, Gil Ad
AU - Malul, Tal
AU - Eliaz, Yossi
AU - Eyal, Eran
AU - Veksler-Lublinsky, Isana
N1 - Publisher Copyright:
© The Author(s) 2023.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - The CRISPR-Cas9 system has been adapted to achieve targeted genome editing as well as transcriptional control by customizing 20-nt guide RNA (gRNA) molecules for desired regions in the target genome. Designing gRNAs must consider nonspecific and unintended binding, known as off-targets, since these may have potentially harmful effects. To assist in gRNA design, we have developed OffRisk. This Docker-based tool annotates off-target sites in the human genome and assigns them a potential risk label by incorporating functional and regulatory information at different molecular levels.
AB - The CRISPR-Cas9 system has been adapted to achieve targeted genome editing as well as transcriptional control by customizing 20-nt guide RNA (gRNA) molecules for desired regions in the target genome. Designing gRNAs must consider nonspecific and unintended binding, known as off-targets, since these may have potentially harmful effects. To assist in gRNA design, we have developed OffRisk. This Docker-based tool annotates off-target sites in the human genome and assigns them a potential risk label by incorporating functional and regulatory information at different molecular levels.
UR - http://www.scopus.com/inward/record.url?scp=85175545625&partnerID=8YFLogxK
U2 - 10.1093/bioadv/vbad138
DO - 10.1093/bioadv/vbad138
M3 - Article
C2 - 37840905
AN - SCOPUS:85175545625
SN - 2635-0041
VL - 3
JO - Bioinformatics Advances
JF - Bioinformatics Advances
IS - 1
M1 - vbad138
ER -