Oligochitosan and oxidized nucleoside-based bioderived hydrogels for wound healing

Rafael Itzhakov, Noy Eretz-Kdosha, Eldad Silberstein, Topaz Alfer, Raanan Gvirtz, Elazar Fallik, Navit Ogen-Shtern, Guy Cohen, Elena Poverenov

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Herein, we report biocompatible hydrogel for wound healing that was prepared using nature-sourced building blocks. For the first time, OCS was employed as a building macromolecule to form bulk hydrogels along with the nature-sourced nucleoside derivative (inosine dialdehyde, IdA) as the cross-linker. A strong correlation was obtained between the mechanical properties and stability of the prepared hydrogels with a cross-linker concentration. The Cryo-SEM images of IdA/OCS hydrogels showed an interconnected spongy-like porous structure. Alexa 555 labeled bovine serum albumin was incorporated into the hydrogels matrix. The release kinetics studies under physiological conditions indicated that cross-linker concentration could also control the release rate. The potential of hydrogels in wound healing applications was tested in vitro and ex vivo on human skin. Topical application of the hydrogel was excellently tolerated by the skin with no impairment of epidermal viability or irritation, determined by MTT and IL-1α assays, respectively. The hydrogels were used to load and deliver epidermal growth factor (EGF), showing an increase in its ameliorating action, effectively enhancing wound closure inflicted by punch biopsy. Furthermore, BrdU incorporation assay performed in both fibroblast and keratinocyte cells revealed an increased proliferation in hydrogel-treated cells and an enhancement of EGF impact in keratinocytes.

Original languageEnglish
Article number120947
JournalCarbohydrate Polymers
Volume314
DOIs
StatePublished - 15 Aug 2023
Externally publishedYes

Keywords

  • Biocompatible cross-linker
  • Ex vivo human skin model
  • Hydrogels
  • Oligochitosan
  • Wound healing

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics
  • Organic Chemistry

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