TY - JOUR
T1 - On the continuous culturing of B.95-8 cells en the presence of phosphonoacetic acid
AU - Margalith, Miriam
AU - Manor, Daphna
AU - Goldblum, Natan
N1 - Funding Information:
*This study was supported by grants from the Stiftung Research Fund and by the Max Bogen Research Fund.
PY - 1980/1/1
Y1 - 1980/1/1
N2 - Lymphoblastoid B.95-8 cells were cultured for four months and three weeks in the presence of increasing concentrations (50-200 μg/ml) of phosphonoacetic acid (PAA). Several weeks after removal of the PAA, the cultures, in parallel with untreated B.95-8 cells, were tested for the presence of: a) Epstein-Barr virus (EBV) viral capsid antigen (VCA), and b) transformation of human cord blood lymphocytes. There was no difference in the percentage of cells exhibiting VCA in the B.95-8 PAA treated and untreated cells. However, transformation assays indicated 10 times less transforming virus in culture supernatant harvested from B.95-8 cultures treated with PAA, as compared with the control cultures. Electron microscopic studies indicated the presence of virus particles in B.95-8 control cells and their almost complete absence in the PAA-treated cells.
AB - Lymphoblastoid B.95-8 cells were cultured for four months and three weeks in the presence of increasing concentrations (50-200 μg/ml) of phosphonoacetic acid (PAA). Several weeks after removal of the PAA, the cultures, in parallel with untreated B.95-8 cells, were tested for the presence of: a) Epstein-Barr virus (EBV) viral capsid antigen (VCA), and b) transformation of human cord blood lymphocytes. There was no difference in the percentage of cells exhibiting VCA in the B.95-8 PAA treated and untreated cells. However, transformation assays indicated 10 times less transforming virus in culture supernatant harvested from B.95-8 cultures treated with PAA, as compared with the control cultures. Electron microscopic studies indicated the presence of virus particles in B.95-8 control cells and their almost complete absence in the PAA-treated cells.
UR - http://www.scopus.com/inward/record.url?scp=0019198044&partnerID=8YFLogxK
U2 - 10.1016/0166-0934(80)90052-X
DO - 10.1016/0166-0934(80)90052-X
M3 - Article
C2 - 6262340
AN - SCOPUS:0019198044
SN - 0166-0934
VL - 1
SP - 349
EP - 354
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 6
ER -