On the Synthesis of Glutathione in Human Blood Cells In Vitro

Mature human blood cells depleted of ATP in vitro by preincubation with fluoride, maintain the ability to incorporate glycine into their GSH, presumably by exchange. The incorporation of labelled glycine into GSH, of ATP depleted blood cells, in the presence of added phosphate, is higher than in the nonpoisoned cells. Arsenate accelerates the incorporation of labelled glycine into GSH of ATP depleted blood cells. The inhibitory effect of glucose on the incorporation of labelled glycine into blood cell GSH is thought to be related to the binding of inorganic phosphate. Synthesis of GSH in mature blood cells in vitro was established with labelled cystein and cystine.