TY - JOUR
T1 - One precursor, three apolipoproteins
T2 - The relationship between two crustacean lipoproteins, the large discoidal lipoprotein and the high density lipoprotein/β-glucan binding protein
AU - Stieb, Stefanie
AU - Roth, Ziv
AU - Dal Magro, Christina
AU - Fischer, Sabine
AU - Butz, Eric
AU - Sagi, Amir
AU - Khalaila, Isam
AU - Lieb, Bernhard
AU - Schenk, Sven
AU - Hoeger, Ulrich
N1 - Publisher Copyright:
© 2014 Elsevier B.V. All rights reserved.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - The novel discoidal lipoprotein (dLp) recently detected in the crayfish, differs from other crustacean lipoproteins in its large size, apoprotein composition and high lipid binding capacity. We identified the dLp sequence by transcriptome analyses of the hepatopancreas and mass spectrometry. Further de novo assembly of the NGS data followed by BLAST searches using the sequence of the high density lipoprotein/β-glucan binding protein (HDL-BGBP) of Astacus leptodactylus as query revealed a putative precursor molecule with an open reading frame of 14.7 kb and a deduced primary structure of 4889 amino acids. The presence of an N-terminal lipid binding domain and a DUF 1943 domain suggests the relationship with the large lipid transfer proteins. Two putative dibasic furin cleavage sites were identified bordering the sequence of the HDL-BGBP. When subjected to mass spectroscopic analyses, tryptic peptides of the large apoprotein of dLp matched the N-terminal part of the precursor, while the peptides obtained for its small apoprotein matched the C-terminal part. Repeating the analysis in the prawn Macrobrachium rosenbergii revealed a similar protein with identical domain architecture suggesting that our findings do not represent an isolated instance. Our results indicate that the above three apolipoproteins (i.e HDL-BGBP and both the large and the small subunit of dLp) are translated as a large precursor. Cleavage at the furin type sites releases two subunits forming a heterodimeric dLP particle, while the remaining part forms an HDL-BGBP whose relationship with other lipoproteins as well as specific functions are yet to be elucidated.
AB - The novel discoidal lipoprotein (dLp) recently detected in the crayfish, differs from other crustacean lipoproteins in its large size, apoprotein composition and high lipid binding capacity. We identified the dLp sequence by transcriptome analyses of the hepatopancreas and mass spectrometry. Further de novo assembly of the NGS data followed by BLAST searches using the sequence of the high density lipoprotein/β-glucan binding protein (HDL-BGBP) of Astacus leptodactylus as query revealed a putative precursor molecule with an open reading frame of 14.7 kb and a deduced primary structure of 4889 amino acids. The presence of an N-terminal lipid binding domain and a DUF 1943 domain suggests the relationship with the large lipid transfer proteins. Two putative dibasic furin cleavage sites were identified bordering the sequence of the HDL-BGBP. When subjected to mass spectroscopic analyses, tryptic peptides of the large apoprotein of dLp matched the N-terminal part of the precursor, while the peptides obtained for its small apoprotein matched the C-terminal part. Repeating the analysis in the prawn Macrobrachium rosenbergii revealed a similar protein with identical domain architecture suggesting that our findings do not represent an isolated instance. Our results indicate that the above three apolipoproteins (i.e HDL-BGBP and both the large and the small subunit of dLp) are translated as a large precursor. Cleavage at the furin type sites releases two subunits forming a heterodimeric dLP particle, while the remaining part forms an HDL-BGBP whose relationship with other lipoproteins as well as specific functions are yet to be elucidated.
KW - Crustacea
KW - Discoidal lipoprotein
KW - High density lipoprotein/-glucan binding protein
KW - Lipoprotein precursor
KW - Transcriptome analysis
UR - http://www.scopus.com/inward/record.url?scp=84908518417&partnerID=8YFLogxK
U2 - 10.1016/j.bbalip.2014.09.020
DO - 10.1016/j.bbalip.2014.09.020
M3 - Article
AN - SCOPUS:84908518417
SN - 1388-1981
VL - 1841
SP - 1700
EP - 1708
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 12
ER -