One step electro-oxidative preparation of graphene quantum dots from wood charcoal as a peroxidase mimetic

Narsingh R. Nirala; Gaurav Khandelwal; Brijesh Kumar; Vinita; Rajiv Prakash; Vinod Kumar

doi:10.1016/j.talanta.2017.05.061

One step electro-oxidative preparation of graphene quantum dots from wood charcoal as a peroxidase mimetic

Narsingh R. Nirala, Gaurav Khandelwal, Brijesh Kumar, Vinita, Rajiv Prakash, Vinod Kumar

Department of Materials Engineering

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

In present study, we highlight one-step electrochemical synthesis of nearly uniform size (~ 5 nm) of graphene quantum dots (E-GQDs) from wood charcoal and their further application as a peroxidase enzyme mimetic. The structural and optical properties of as-synthesized E-GQDs were probed by TEM, AFM, and spectroscopic techniques. Peroxidase enzyme mimetic potential of E-GQDs were examined for colorimetric detection of H₂O₂ and glucose. E-GQDs allowed a rapid and sensitive detection of glucose with a detection limit of 0.006 mM for dynamic response range of 0.01–0.6 mM. The calculated higher value of V_max (7.2 × 10⁻⁷ M s⁻¹) along with lower K_m (0.012 mM) corroborate enhanced the peroxidase-like activity of E-GQDs. Study introduces a cheap and widely available raw material for the electrochemical synthesis of graphene quantum dots with commendable enzyme mimetic activity which may have a huge impact in developing calorimetric bioanalysis systems.

Original language	English
Pages (from-to)	36-43
Number of pages	8
Journal	Talanta
Volume	173
DOIs	https://doi.org/10.1016/j.talanta.2017.05.061
State	Published - 1 Oct 2017

Keywords

Colorimetric
E-GQDs
Glucose oxidase (GOx)
Horse radish peroxidase (HRP)
Peroxidase mimetic

ASJC Scopus subject areas

Analytical Chemistry

Access to Document

10.1016/j.talanta.2017.05.061

Cite this

@article{7045f34e3b8a4b128e9e99222c0e3191,

title = "One step electro-oxidative preparation of graphene quantum dots from wood charcoal as a peroxidase mimetic",

abstract = "In present study, we highlight one-step electrochemical synthesis of nearly uniform size (~ 5 nm) of graphene quantum dots (E-GQDs) from wood charcoal and their further application as a peroxidase enzyme mimetic. The structural and optical properties of as-synthesized E-GQDs were probed by TEM, AFM, and spectroscopic techniques. Peroxidase enzyme mimetic potential of E-GQDs were examined for colorimetric detection of H2O2 and glucose. E-GQDs allowed a rapid and sensitive detection of glucose with a detection limit of 0.006 mM for dynamic response range of 0.01–0.6 mM. The calculated higher value of Vmax (7.2 × 10−7 M s−1) along with lower Km (0.012 mM) corroborate enhanced the peroxidase-like activity of E-GQDs. Study introduces a cheap and widely available raw material for the electrochemical synthesis of graphene quantum dots with commendable enzyme mimetic activity which may have a huge impact in developing calorimetric bioanalysis systems.",

keywords = "Colorimetric, E-GQDs, Glucose oxidase (GOx), Horse radish peroxidase (HRP), Peroxidase mimetic",

author = "Nirala, {Narsingh R.} and Gaurav Khandelwal and Brijesh Kumar and Vinita and Rajiv Prakash and Vinod Kumar",

note = "Publisher Copyright: {\textcopyright} 2017 Elsevier B.V.",

year = "2017",

month = oct,

day = "1",

doi = "10.1016/j.talanta.2017.05.061",

language = "English",

volume = "173",

pages = "36--43",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - One step electro-oxidative preparation of graphene quantum dots from wood charcoal as a peroxidase mimetic

AU - Nirala, Narsingh R.

AU - Khandelwal, Gaurav

AU - Kumar, Brijesh

AU - Vinita,

AU - Prakash, Rajiv

AU - Kumar, Vinod

PY - 2017/10/1

Y1 - 2017/10/1

N2 - In present study, we highlight one-step electrochemical synthesis of nearly uniform size (~ 5 nm) of graphene quantum dots (E-GQDs) from wood charcoal and their further application as a peroxidase enzyme mimetic. The structural and optical properties of as-synthesized E-GQDs were probed by TEM, AFM, and spectroscopic techniques. Peroxidase enzyme mimetic potential of E-GQDs were examined for colorimetric detection of H2O2 and glucose. E-GQDs allowed a rapid and sensitive detection of glucose with a detection limit of 0.006 mM for dynamic response range of 0.01–0.6 mM. The calculated higher value of Vmax (7.2 × 10−7 M s−1) along with lower Km (0.012 mM) corroborate enhanced the peroxidase-like activity of E-GQDs. Study introduces a cheap and widely available raw material for the electrochemical synthesis of graphene quantum dots with commendable enzyme mimetic activity which may have a huge impact in developing calorimetric bioanalysis systems.

AB - In present study, we highlight one-step electrochemical synthesis of nearly uniform size (~ 5 nm) of graphene quantum dots (E-GQDs) from wood charcoal and their further application as a peroxidase enzyme mimetic. The structural and optical properties of as-synthesized E-GQDs were probed by TEM, AFM, and spectroscopic techniques. Peroxidase enzyme mimetic potential of E-GQDs were examined for colorimetric detection of H2O2 and glucose. E-GQDs allowed a rapid and sensitive detection of glucose with a detection limit of 0.006 mM for dynamic response range of 0.01–0.6 mM. The calculated higher value of Vmax (7.2 × 10−7 M s−1) along with lower Km (0.012 mM) corroborate enhanced the peroxidase-like activity of E-GQDs. Study introduces a cheap and widely available raw material for the electrochemical synthesis of graphene quantum dots with commendable enzyme mimetic activity which may have a huge impact in developing calorimetric bioanalysis systems.

KW - Colorimetric

KW - E-GQDs

KW - Glucose oxidase (GOx)

KW - Horse radish peroxidase (HRP)

KW - Peroxidase mimetic

UR - http://www.scopus.com/inward/record.url?scp=85019657885&partnerID=8YFLogxK

U2 - 10.1016/j.talanta.2017.05.061

DO - 10.1016/j.talanta.2017.05.061

M3 - Article

C2 - 28602189

AN - SCOPUS:85019657885

SN - 0039-9140

VL - 173

SP - 36

EP - 43

JO - Talanta

JF - Talanta

ER -

One step electro-oxidative preparation of graphene quantum dots from wood charcoal as a peroxidase mimetic

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this