“On/off/on” hydrogen-peroxide sensor with hemoglobin-functionalized carbon dots

Susanta Kumar Bhunia, Susmita Dolai, Hongchen Sun, Raz Jelinek

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


We demonstrate a new scheme for hydrogen peroxide (H2O2) detection through a distinctive fluorescence “on/off/on” mechanism. Specifically, we show that the fluorescence emission of carbon dots (C-dots) prepared from L-penicillamine (LPA) as the carbon precursor underwent initial quenching upon addition of hemoglobin (“off” state) but could be completely recovered upon addition of H2O2 (fluorescence “on”). The “on/off/on” phenomenon was observed only in case of C-dots prepared from penicillamine, underscoring a unique radical-scavenging mechanism by thiol residues upon the C-dots’ surface. This study illustrates a novel C-dot fluorescence tuning through heme oxidation and demonstrates a useful sensing modality for oxidative targets.

Original languageEnglish
Pages (from-to)223-230
Number of pages8
JournalSensors and Actuators, B: Chemical
StatePublished - 1 Oct 2018


  • Carbon dots
  • Fluorescence quenching
  • Hemoglobin
  • Hydrogen peroxide sensor
  • π − π Interactions


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