Opioids modulate interleukin-1 production and secretion by bone-marrow macrophages

Ron N. Apte; Scott K. Durum; Joost J. Oppenheim

doi:10.1016/0165-2478(90)90026-M

Opioids modulate interleukin-1 production and secretion by bone-marrow macrophages

Ron N. Apte, Scott K. Durum, Joost J. Oppenheim

The Shraga Segal Department of Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

In the present study, we have assessed the effect of opioids (endorphins, enkephalins and neoendorphins) on production of IL-1 activity by bone-marrow-derived macrophages. None of the neuropeptides induced IL-1 production by itself. However, some of the opioids potentiated IL-1 production and release in macrophages concomitantly stimulated by lipopolysaccharide (LPS) or silica. LPS induced predominantly intracellular IL-1 activity, whereas most of the silica-induced IL-1 was released extracellularly. β-Endorphin, leucin-enkephalin (leu-enkephalin) and β-neoendorphin all potentiated both intracellular and extracellular release of IL 1 induced by either LPS or silica. In contrast, α-endorphin, methionine-enkephalin (metenkephalin) and α-neoendorphin did not influence IL-1 production or release. The potentiating effects of β-endorphin on LPS-induced IL-1 production/secretion were inhibited by naloxone, pointing to an involvement of opioid receptors.

Original language	English
Pages (from-to)	141-148
Number of pages	8
Journal	Immunology Letters
Volume	24
Issue number	2
DOIs	https://doi.org/10.1016/0165-2478(90)90026-M
State	Published - 1 Jan 1990

Keywords

Bone marrow
Interleukin-1
Macrophage
Opioids

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0165-2478(90)90026-M

Cite this

@article{ca4a6795d34e4808bdf20dca385bcd89,

title = "Opioids modulate interleukin-1 production and secretion by bone-marrow macrophages",

abstract = "In the present study, we have assessed the effect of opioids (endorphins, enkephalins and neoendorphins) on production of IL-1 activity by bone-marrow-derived macrophages. None of the neuropeptides induced IL-1 production by itself. However, some of the opioids potentiated IL-1 production and release in macrophages concomitantly stimulated by lipopolysaccharide (LPS) or silica. LPS induced predominantly intracellular IL-1 activity, whereas most of the silica-induced IL-1 was released extracellularly. β-Endorphin, leucin-enkephalin (leu-enkephalin) and β-neoendorphin all potentiated both intracellular and extracellular release of IL 1 induced by either LPS or silica. In contrast, α-endorphin, methionine-enkephalin (metenkephalin) and α-neoendorphin did not influence IL-1 production or release. The potentiating effects of β-endorphin on LPS-induced IL-1 production/secretion were inhibited by naloxone, pointing to an involvement of opioid receptors.",

keywords = "Bone marrow, Interleukin-1, Macrophage, Opioids",

author = "Apte, {Ron N.} and Durum, {Scott K.} and Oppenheim, {Joost J.}",

note = "Funding Information: The authors would like to thank Dr. Lily Neumann, (Department of Epidemiology, Faculty of Health Sciences, Ben Gurion University) for assistance in the statistical analyses of the results, Drs. William Farrar, Ruth Neta and Dan Longo for critical review of the paper, and Mrs. Caroline Simon and Mrs. Bobbie Unger for assistance in preparing the manuscript. This project was supported in part by grants awarded to R.N.A. by the Basic Research Fund of the Israel Academy of Sciences and Humanities and the Doron Foundation. Funding Information: Note.{"} This project has been funded at least in part with Federal funds from the Dept. of Health and Human Services under contract NO1-CO-74102 with Program Resources, Inc. The content of this publication does not necessarily reflect the view or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.",

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doi = "10.1016/0165-2478(90)90026-M",

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T1 - Opioids modulate interleukin-1 production and secretion by bone-marrow macrophages

AU - Apte, Ron N.

AU - Durum, Scott K.

AU - Oppenheim, Joost J.

N1 - Funding Information: The authors would like to thank Dr. Lily Neumann, (Department of Epidemiology, Faculty of Health Sciences, Ben Gurion University) for assistance in the statistical analyses of the results, Drs. William Farrar, Ruth Neta and Dan Longo for critical review of the paper, and Mrs. Caroline Simon and Mrs. Bobbie Unger for assistance in preparing the manuscript. This project was supported in part by grants awarded to R.N.A. by the Basic Research Fund of the Israel Academy of Sciences and Humanities and the Doron Foundation. Funding Information: Note." This project has been funded at least in part with Federal funds from the Dept. of Health and Human Services under contract NO1-CO-74102 with Program Resources, Inc. The content of this publication does not necessarily reflect the view or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - In the present study, we have assessed the effect of opioids (endorphins, enkephalins and neoendorphins) on production of IL-1 activity by bone-marrow-derived macrophages. None of the neuropeptides induced IL-1 production by itself. However, some of the opioids potentiated IL-1 production and release in macrophages concomitantly stimulated by lipopolysaccharide (LPS) or silica. LPS induced predominantly intracellular IL-1 activity, whereas most of the silica-induced IL-1 was released extracellularly. β-Endorphin, leucin-enkephalin (leu-enkephalin) and β-neoendorphin all potentiated both intracellular and extracellular release of IL 1 induced by either LPS or silica. In contrast, α-endorphin, methionine-enkephalin (metenkephalin) and α-neoendorphin did not influence IL-1 production or release. The potentiating effects of β-endorphin on LPS-induced IL-1 production/secretion were inhibited by naloxone, pointing to an involvement of opioid receptors.

AB - In the present study, we have assessed the effect of opioids (endorphins, enkephalins and neoendorphins) on production of IL-1 activity by bone-marrow-derived macrophages. None of the neuropeptides induced IL-1 production by itself. However, some of the opioids potentiated IL-1 production and release in macrophages concomitantly stimulated by lipopolysaccharide (LPS) or silica. LPS induced predominantly intracellular IL-1 activity, whereas most of the silica-induced IL-1 was released extracellularly. β-Endorphin, leucin-enkephalin (leu-enkephalin) and β-neoendorphin all potentiated both intracellular and extracellular release of IL 1 induced by either LPS or silica. In contrast, α-endorphin, methionine-enkephalin (metenkephalin) and α-neoendorphin did not influence IL-1 production or release. The potentiating effects of β-endorphin on LPS-induced IL-1 production/secretion were inhibited by naloxone, pointing to an involvement of opioid receptors.

KW - Bone marrow

KW - Interleukin-1

KW - Macrophage

KW - Opioids

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DO - 10.1016/0165-2478(90)90026-M

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AN - SCOPUS:0025280332

SN - 0165-2478

VL - 24

SP - 141

EP - 148

JO - Immunology Letters

JF - Immunology Letters

IS - 2

ER -

Opioids modulate interleukin-1 production and secretion by bone-marrow macrophages

Abstract

Keywords

ASJC Scopus subject areas

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