Optical detection of lead and potassium ions using a quantum-dot-based aptamer nanosensor

Xenia Meshik; Ke Xu; Mitra Dutta; Michael A. Stroscio

doi:10.1109/TNB.2014.2317315

Optical detection of lead and potassium ions using a quantum-dot-based aptamer nanosensor

Xenia Meshik, Ke Xu, Mitra Dutta, Michael A. Stroscio

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Quantum-dot (QD) based nanosensors can be used to detect a wide range of molecules. This study examined a nanosensor comprised of thrombin binding aptamer (TBA) with 700NC InGaP QD on the 5' terminus and an Au nanoparticle quencher on the 3' terminus. Both K⁺ and Pb²⁺ bind to TBA, resulting in a conformational change that brings the Au quencher closer to the QD. Photoluminescence measurements indicated a decrease in fluorescence corresponding to an increase in either K⁺ or Pb²⁺ concentration. For healthy blood serum K⁺ concentrations (3.5-5 mM), the beacon exhibited 15-17% quenching efficiency. Pb²⁺ concentration of 0.48 μM, the threshold for toxicity in serum, yielded 14% quenching. The beacon's ability to detect changes in ion levels in a critical range of concentrations can make it an effective diagnostic tool.

Original language	English
Article number	6804009
Pages (from-to)	161-164
Number of pages	4
Journal	IEEE Transactions on Nanobioscience
Volume	13
Issue number	2
DOIs	https://doi.org/10.1109/TNB.2014.2317315
State	Published - 1 Jan 2014
Externally published	Yes

Keywords

DNA
gold nanoparticles
ion detection
photoluminescence
quantum dots (QD)
thrombin binding aptamer (TBA)

ASJC Scopus subject areas

Biotechnology
Medicine (miscellaneous)
Bioengineering
Biomedical Engineering
Pharmaceutical Science
Computer Science Applications
Electrical and Electronic Engineering

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10.1109/TNB.2014.2317315

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abstract = "Quantum-dot (QD) based nanosensors can be used to detect a wide range of molecules. This study examined a nanosensor comprised of thrombin binding aptamer (TBA) with 700NC InGaP QD on the 5' terminus and an Au nanoparticle quencher on the 3' terminus. Both K+ and Pb2+ bind to TBA, resulting in a conformational change that brings the Au quencher closer to the QD. Photoluminescence measurements indicated a decrease in fluorescence corresponding to an increase in either K+ or Pb2+ concentration. For healthy blood serum K+ concentrations (3.5-5 mM), the beacon exhibited 15-17% quenching efficiency. Pb2+ concentration of 0.48 μM, the threshold for toxicity in serum, yielded 14% quenching. The beacon's ability to detect changes in ion levels in a critical range of concentrations can make it an effective diagnostic tool.",

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AU - Dutta, Mitra

AU - Stroscio, Michael A.

PY - 2014/1/1

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N2 - Quantum-dot (QD) based nanosensors can be used to detect a wide range of molecules. This study examined a nanosensor comprised of thrombin binding aptamer (TBA) with 700NC InGaP QD on the 5' terminus and an Au nanoparticle quencher on the 3' terminus. Both K+ and Pb2+ bind to TBA, resulting in a conformational change that brings the Au quencher closer to the QD. Photoluminescence measurements indicated a decrease in fluorescence corresponding to an increase in either K+ or Pb2+ concentration. For healthy blood serum K+ concentrations (3.5-5 mM), the beacon exhibited 15-17% quenching efficiency. Pb2+ concentration of 0.48 μM, the threshold for toxicity in serum, yielded 14% quenching. The beacon's ability to detect changes in ion levels in a critical range of concentrations can make it an effective diagnostic tool.

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Optical detection of lead and potassium ions using a quantum-dot-based aptamer nanosensor

Abstract

Keywords

ASJC Scopus subject areas

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