Optical fiber bioluminescent whole-cell microbial biosensors to genotoxicants

B. Polyak, E. Bassis, A. Novodvorets, S. Belkin, R. S. Marks

    Research output: Contribution to journalConference articlepeer-review

    70 Scopus citations

    Abstract

    Conservation of water resources calls for ever stricter regulatory measures and better monitoring systems. Whole-cell bacterial sensors have been genetically engineered to react to target toxicants by the induction of a selected promoter and the subsequent production of bioluminescent light through a recombinant lux reporter. In order to create a one-step assay, we have designed a new, self-contained, disposable optical fiber sensor module and a customized photodetector system that integrates these microorganisms. A photon-counting photomultiplier tube-based instrument was constructed. Optical fiber tip cores were covered with adlayer films consisting of calcium alginate containing bioluminescent bacterial sensors of genotoxicants. Multiplying these steps thickened the adlayer in increments, increasing the number of bacterial reporters attached to the optical fiber transducer. These whole cell optrodes are responsive to external traces of DNA damaging agents such as mitomycin C. Light production was shown to be dose-dependent and proportional to the number of bacterial layers.

    Original languageEnglish
    Pages (from-to)305-311
    Number of pages7
    JournalWater Science and Technology
    Volume42
    Issue number1-2
    DOIs
    StatePublished - 1 Jan 2000
    EventProceedings of the 7th International Conference of the Israel Society for Ecology and Environmental Quality Sciences - Jerusalem, Isr
    Duration: 13 Jun 199918 Jun 1999

    Keywords

    • Bioluminescent bacteria
    • Biosensor
    • Environmental monitoring
    • Genotoxicity
    • Mitomycin C
    • Optical fiber
    • Sodium alginate

    ASJC Scopus subject areas

    • Environmental Engineering
    • Water Science and Technology

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