Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans

A. Sobarzo, J. T. Paweska, S. Herrmann, T. Amir, R. S. Marks, L. Lobel

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

This paper describes the development and evaluation of an optical fiber immunosensor (OFIS) for the detection of IgG antibody to Rift Valley fever virus (RVFV) in humans. The OFIS was based on a sandwich enzyme-linked immunosorbent assay (S-ELISA) format, whereby gamma-irradiated RVFV and control antigens were immobilized on the optical fiber surface coated with a mouse anti-RVFV antibody. Data sets derived from field-collected sera in Africa (n = 242) were dichotomized according to the results of a virus neutralization test. Compared to standard colorimetric S-ELISA, the OFIS technique was more sensitive in detecting smaller quantity of specific IgG to RVFV in human sera. At cut-off value selected at a 95% accuracy level by the two-graph receiver operating characteristic analysis, the OFIS diagnostic sensitivity was 97.22% and diagnostic specificity 98.86%. Our results demonstrate that the OFIS technology reported here is highly accurate, simple to perform and has the potential to be used in a portable format.

Original languageEnglish
Pages (from-to)327-334
Number of pages8
JournalJournal of Virological Methods
Volume146
Issue number1-2
DOIs
StatePublished - 1 Dec 2007

Keywords

  • Humans
  • IgG
  • Optical fiber immunosensor
  • Rift Valley fever virus

ASJC Scopus subject areas

  • Virology

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