Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans

A. Sobarzo; J. T. Paweska; S. Herrmann; T. Amir; R. S. Marks; L. Lobel

doi:10.1016/j.jviromet.2007.07.017

Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans

A. Sobarzo, J. T. Paweska, S. Herrmann, T. Amir, R. S. Marks, L. Lobel

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

This paper describes the development and evaluation of an optical fiber immunosensor (OFIS) for the detection of IgG antibody to Rift Valley fever virus (RVFV) in humans. The OFIS was based on a sandwich enzyme-linked immunosorbent assay (S-ELISA) format, whereby gamma-irradiated RVFV and control antigens were immobilized on the optical fiber surface coated with a mouse anti-RVFV antibody. Data sets derived from field-collected sera in Africa (n = 242) were dichotomized according to the results of a virus neutralization test. Compared to standard colorimetric S-ELISA, the OFIS technique was more sensitive in detecting smaller quantity of specific IgG to RVFV in human sera. At cut-off value selected at a 95% accuracy level by the two-graph receiver operating characteristic analysis, the OFIS diagnostic sensitivity was 97.22% and diagnostic specificity 98.86%. Our results demonstrate that the OFIS technology reported here is highly accurate, simple to perform and has the potential to be used in a portable format.

Original language	English
Pages (from-to)	327-334
Number of pages	8
Journal	Journal of Virological Methods
Volume	146
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2007.07.017
State	Published - 1 Dec 2007

Keywords

Humans
IgG
Optical fiber immunosensor
Rift Valley fever virus

ASJC Scopus subject areas

Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jviromet.2007.07.017

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@article{e4751e8de2564d4bb66b5739ce4677da,

title = "Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans",

abstract = "This paper describes the development and evaluation of an optical fiber immunosensor (OFIS) for the detection of IgG antibody to Rift Valley fever virus (RVFV) in humans. The OFIS was based on a sandwich enzyme-linked immunosorbent assay (S-ELISA) format, whereby gamma-irradiated RVFV and control antigens were immobilized on the optical fiber surface coated with a mouse anti-RVFV antibody. Data sets derived from field-collected sera in Africa (n = 242) were dichotomized according to the results of a virus neutralization test. Compared to standard colorimetric S-ELISA, the OFIS technique was more sensitive in detecting smaller quantity of specific IgG to RVFV in human sera. At cut-off value selected at a 95% accuracy level by the two-graph receiver operating characteristic analysis, the OFIS diagnostic sensitivity was 97.22% and diagnostic specificity 98.86%. Our results demonstrate that the OFIS technology reported here is highly accurate, simple to perform and has the potential to be used in a portable format.",

keywords = "Humans, IgG, Optical fiber immunosensor, Rift Valley fever virus",

author = "A. Sobarzo and Paweska, {J. T.} and S. Herrmann and T. Amir and Marks, {R. S.} and L. Lobel",

note = "Funding Information: We thank P. Leman (SPU-NICD/NHLS) and Y. Libes (BGU) for their excellent technical assistance. We express our gratitude for financial assistance towards this research to the SPU-NICD/NHLS, the Yeshaya Horowitz Association for the BioPen project and the EC6th Framework under the DENFRAME program # 517711.",

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T1 - Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans

AU - Sobarzo, A.

AU - Paweska, J. T.

AU - Herrmann, S.

AU - Amir, T.

AU - Marks, R. S.

AU - Lobel, L.

N1 - Funding Information: We thank P. Leman (SPU-NICD/NHLS) and Y. Libes (BGU) for their excellent technical assistance. We express our gratitude for financial assistance towards this research to the SPU-NICD/NHLS, the Yeshaya Horowitz Association for the BioPen project and the EC6th Framework under the DENFRAME program # 517711.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - This paper describes the development and evaluation of an optical fiber immunosensor (OFIS) for the detection of IgG antibody to Rift Valley fever virus (RVFV) in humans. The OFIS was based on a sandwich enzyme-linked immunosorbent assay (S-ELISA) format, whereby gamma-irradiated RVFV and control antigens were immobilized on the optical fiber surface coated with a mouse anti-RVFV antibody. Data sets derived from field-collected sera in Africa (n = 242) were dichotomized according to the results of a virus neutralization test. Compared to standard colorimetric S-ELISA, the OFIS technique was more sensitive in detecting smaller quantity of specific IgG to RVFV in human sera. At cut-off value selected at a 95% accuracy level by the two-graph receiver operating characteristic analysis, the OFIS diagnostic sensitivity was 97.22% and diagnostic specificity 98.86%. Our results demonstrate that the OFIS technology reported here is highly accurate, simple to perform and has the potential to be used in a portable format.

AB - This paper describes the development and evaluation of an optical fiber immunosensor (OFIS) for the detection of IgG antibody to Rift Valley fever virus (RVFV) in humans. The OFIS was based on a sandwich enzyme-linked immunosorbent assay (S-ELISA) format, whereby gamma-irradiated RVFV and control antigens were immobilized on the optical fiber surface coated with a mouse anti-RVFV antibody. Data sets derived from field-collected sera in Africa (n = 242) were dichotomized according to the results of a virus neutralization test. Compared to standard colorimetric S-ELISA, the OFIS technique was more sensitive in detecting smaller quantity of specific IgG to RVFV in human sera. At cut-off value selected at a 95% accuracy level by the two-graph receiver operating characteristic analysis, the OFIS diagnostic sensitivity was 97.22% and diagnostic specificity 98.86%. Our results demonstrate that the OFIS technology reported here is highly accurate, simple to perform and has the potential to be used in a portable format.

KW - Humans

KW - IgG

KW - Optical fiber immunosensor

KW - Rift Valley fever virus

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Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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