Optical immunosensor for endocrine disruptor nanolayer detection by surface plasmon resonance imaging

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Scopus citations

Abstract

Endocrine disrupting compounds (EDCs) such as bisphenol A (BPA) and female hormone Estrone are especially prevalent in surface and waste-waters in nano-molar concentrations and therefore, there is a need for sensitive analytical device for their monitoring. We have designed a miniature, low cost and fast surface plasmon resonance (SPR) imaging liquid sensor based on the angular interrogation using Kretschmann configuration with diverged incident monochromatic light. During this paper we present a surface plasmon resonance imaging (SPRI) biosensor to detect EDCs such as BPA and estrone. A pattern of SPR line which is dark intensity line on bright area was reflected at angles range depending on the dielectric constant of the analye: Rabbit Anti-Estrone polyclonal IgG + Estrone 11-MUA attached to the silver or non-specific sensing of BPA in water with nanoprecision. For analyzing the SPR signals we used an efficient detection algorithm based on Radon Transform with less sensitivity to laser speckle noise and nonuniformity of the illumination.

Original languageEnglish
Title of host publicationBiosensing and Nanomedicine IV
DOIs
StatePublished - 19 Oct 2011
EventBiosensing and Nanomedicine IV - San Diego, CA, United States
Duration: 21 Aug 201123 Aug 2011

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8099
ISSN (Print)0277-786X

Conference

ConferenceBiosensing and Nanomedicine IV
Country/TerritoryUnited States
CitySan Diego, CA
Period21/08/1123/08/11

Keywords

  • Biosensing
  • Bisphenol A
  • Estrogenic disruptors
  • Estrone
  • Surface Plasmon Resonance (SPR) Imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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