Optical tweezers assisted imaging of the Z-ring in Escherichia coli: Measuring its radial width

G. Carmon, P. Kumar, M. Feingold

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Using single-beam, oscillating optical tweezers we can trap and rotate rod-shaped bacterial cells with respect to the optical axis. This technique allows imaging fluorescently labeled three-dimensional sub-cellular structures from different, optimized viewpoints. To illustrate our method we measure D, the radial width of the Z-ring in unconstricted Escherichia coli. We use cells that express FtsZ-GFP and have their cytoplasmic membrane stained with FM4-64. In a vertically oriented cell, both the Z-ring and the cytoplasmic membrane images appear as symmetric circular structures that lend themselves to quantitative analysis. We found that D ≅ 100 nm, much larger than expected.

Original languageEnglish
Article number013043
JournalNew Journal of Physics
Volume16
DOIs
StatePublished - 1 Jan 2014

ASJC Scopus subject areas

  • Physics and Astronomy (all)

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