Optimization of anastomotic technique and gastric conduit perfusion with hyperspectral imaging and machine learning in an experimental model for minimally invasive esophagectomy

F. Nickel, A. Studier-Fischer, B. Özdemir, J. Odenthal, L. R. Müller, S. Knoedler, K. F. Kowalewski, I. Camplisson, M. M. Allers, M. Dietrich, K. Schmidt, G. A. Salg, H. G. Kenngott, A. T. Billeter, I. Gockel, C. Sagiv, O. E. Hadar, J. Gildenblat, L. Ayala, S. SeidlitzL. Maier-Hein, B. P. Müller-Stich

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Introduction: Esophagectomy is the mainstay of esophageal cancer treatment, but anastomotic insufficiency related morbidity and mortality remain challenging for patient outcome. Therefore, the objective of this work was to optimize anastomotic technique and gastric conduit perfusion with hyperspectral imaging (HSI) for total minimally invasive esophagectomy (MIE) with linear stapled anastomosis. Material and methods: A live porcine model (n = 58) for MIE was used with gastric conduit formation and simulation of linear stapled side-to-side esophagogastrostomy. Four main experimental groups differed in stapling length (3 vs. 6 cm) and simulation of anastomotic position on the conduit (cranial vs. caudal). Tissue oxygenation around the anastomotic simulation site was evaluated using HSI and was validated with histopathology. Results: The tissue oxygenation (ΔStO2) after the anastomotic simulation remained constant only for the short stapler in caudal position (−0.4 ± 4.4%, n.s.) while it was impaired markedly in the other groups (short-cranial: −15.6 ± 11.5%, p = 0.0002; long-cranial: −20.4 ± 7.6%, p = 0.0126; long-caudal: −16.1 ± 9.4%, p < 0.0001). Tissue samples from avascular stomach as measured by HSI showed correspondent eosinophilic pre-necrotic changes in 35.7 ± 9.7% of the surface area. Conclusion: Tissue oxygenation at the site of anastomotic simulation of the gastric conduit during MIE is influenced by stapling technique. Optimal oxygenation was achieved with a short stapler (3 cm) and sufficient distance of the simulated anastomosis to the cranial end of the gastric conduit. HSI tissue deoxygenation corresponded to histopathologic necrotic tissue changes. The experimental model with HSI and ML allow for systematic optimization of gastric conduit perfusion and anastomotic technique while clinical translation will have to be proven.

Original languageEnglish
JournalEuropean Journal of Surgical Oncology
DOIs
StateAccepted/In press - 1 Jan 2023
Externally publishedYes

Keywords

  • Gastric conduit
  • Hyperspectral imaging
  • Linear stapled anastomosis
  • Minimally invasive esophagectomy
  • Porcine model
  • Simulation
  • Tissue perfusion

ASJC Scopus subject areas

  • Oncology
  • Surgery

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