TY - JOUR
T1 - ORG-2058 as a ligand in the assay of progesterone receptor in breast cancer
AU - Sharoni, Yoav
AU - Feldman, Bianca
AU - Karny, Noga
AU - Levy, Joseph
N1 - Funding Information:
This work was supported in part by the Beatty Malkin Grant for cancer research and grants from the Chief Scientist's Office, Israeli Ministry of flealth.and the Israel Cancer Association. The authors wish to thank Mrs. M. Duman for her excellent editorial assistance.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Tritiated [(16α-ethyl-21-hydroxy-19-nor-pregn-4-ene-3, 20-dione)-6,7-3H] (ORG-2058) and 17,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione (R5020) were compared as ligands in the assay of progesterone receptor in human of and rat breast tumors. We found that ORG-2058 is a better ligand because its low nonspecific binding. Most of the nonspecific binding of the other ligand R5020, is to proteins which bind corticosteroids. In cancerous tissue ORG-2058 binds to progesterone receptor linearly in a range of protein concentrations which are normally used in the receptor assay. On the other hand, R5020 exhibits binding linearity over a narrower protein concentration in many tumor biopsies, which may cause severe limitation in the assay procedure or frequent underestimation of receptor content.
AB - Tritiated [(16α-ethyl-21-hydroxy-19-nor-pregn-4-ene-3, 20-dione)-6,7-3H] (ORG-2058) and 17,21-dimethyl-19-nor-pregna-4,9-diene-3,20-dione (R5020) were compared as ligands in the assay of progesterone receptor in human of and rat breast tumors. We found that ORG-2058 is a better ligand because its low nonspecific binding. Most of the nonspecific binding of the other ligand R5020, is to proteins which bind corticosteroids. In cancerous tissue ORG-2058 binds to progesterone receptor linearly in a range of protein concentrations which are normally used in the receptor assay. On the other hand, R5020 exhibits binding linearity over a narrower protein concentration in many tumor biopsies, which may cause severe limitation in the assay procedure or frequent underestimation of receptor content.
UR - http://www.scopus.com/inward/record.url?scp=0022944888&partnerID=8YFLogxK
U2 - 10.1016/0039-128X(86)90028-0
DO - 10.1016/0039-128X(86)90028-0
M3 - Article
AN - SCOPUS:0022944888
SN - 0039-128X
VL - 48
SP - 419
EP - 426
JO - Steroids
JF - Steroids
IS - 5-6
ER -