Organogenesis in the vine cactus Selenicereus megalanthus using thidiazuron

D. Pelah; R. A. Kaushik; Y. Mizrahi; Y. Sitrit

doi:10.1023/A:1016585108094

Organogenesis in the vine cactus Selenicereus megalanthus using thidiazuron

D. Pelah, R. A. Kaushik, Y. Mizrahi, Y. Sitrit

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Segments of distal and proximal parts of the cotyledons, hypocotyls and epicotyls of yellow pitaya [Selenicereus megalanthus (Cactaceae)], were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). The proximal part of the cotyledons was found to be the most responsive tissue, maximal bud regeneration being achieved in the presence of 200 μM thidiazuron. However, TDZ does not change the pattern of regeneration competence, which remains restricted to the proximal part of the cotyledons. The best medium for shoot was found to be MS lacking plant growth regulators. Rooting was induced on MS medium supplemented with 5.3 μM naphthaleneacetic acid. Rooted plantlets were transferred to a soil medium and acclimatized successfully in a greenhouse.

Original language	English
Pages (from-to)	81-84
Number of pages	4
Journal	Plant Cell, Tissue and Organ Culture
Volume	71
Issue number	1
DOIs	https://doi.org/10.1023/A:1016585108094
State	Published - 1 Oct 2002

Keywords

Cacti
Plant regeneration
Thidiazuron (TDZ)
Tissue culture
Yellow pitaya

ASJC Scopus subject areas

Horticulture

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10.1023/A:1016585108094

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abstract = "Segments of distal and proximal parts of the cotyledons, hypocotyls and epicotyls of yellow pitaya [Selenicereus megalanthus (Cactaceae)], were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). The proximal part of the cotyledons was found to be the most responsive tissue, maximal bud regeneration being achieved in the presence of 200 μM thidiazuron. However, TDZ does not change the pattern of regeneration competence, which remains restricted to the proximal part of the cotyledons. The best medium for shoot was found to be MS lacking plant growth regulators. Rooting was induced on MS medium supplemented with 5.3 μM naphthaleneacetic acid. Rooted plantlets were transferred to a soil medium and acclimatized successfully in a greenhouse.",

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T1 - Organogenesis in the vine cactus Selenicereus megalanthus using thidiazuron

AU - Pelah, D.

AU - Kaushik, R. A.

AU - Mizrahi, Y.

AU - Sitrit, Y.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Segments of distal and proximal parts of the cotyledons, hypocotyls and epicotyls of yellow pitaya [Selenicereus megalanthus (Cactaceae)], were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). The proximal part of the cotyledons was found to be the most responsive tissue, maximal bud regeneration being achieved in the presence of 200 μM thidiazuron. However, TDZ does not change the pattern of regeneration competence, which remains restricted to the proximal part of the cotyledons. The best medium for shoot was found to be MS lacking plant growth regulators. Rooting was induced on MS medium supplemented with 5.3 μM naphthaleneacetic acid. Rooted plantlets were transferred to a soil medium and acclimatized successfully in a greenhouse.

AB - Segments of distal and proximal parts of the cotyledons, hypocotyls and epicotyls of yellow pitaya [Selenicereus megalanthus (Cactaceae)], were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). The proximal part of the cotyledons was found to be the most responsive tissue, maximal bud regeneration being achieved in the presence of 200 μM thidiazuron. However, TDZ does not change the pattern of regeneration competence, which remains restricted to the proximal part of the cotyledons. The best medium for shoot was found to be MS lacking plant growth regulators. Rooting was induced on MS medium supplemented with 5.3 μM naphthaleneacetic acid. Rooted plantlets were transferred to a soil medium and acclimatized successfully in a greenhouse.

KW - Cacti

KW - Plant regeneration

KW - Thidiazuron (TDZ)

KW - Tissue culture

KW - Yellow pitaya

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Organogenesis in the vine cactus Selenicereus megalanthus using thidiazuron

Abstract

Keywords

ASJC Scopus subject areas

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