ORTHOGONAL TRANSLATION COMPONENTS FOR THE IN VIVO INCORPORATION OF UNNATURAL AMINO ACIDS

Peter SCHULTZ; Lital Alfonta; Jonathan R Chittuluru; Alexander Deiters; Dan Groff; Daniel Summerer; Meng-Lin Tsao; Jiangyun Wang; Ning Wu; Jianming Xie; Huaqiang. Zeng

ORTHOGONAL TRANSLATION COMPONENTS FOR THE IN VIVO INCORPORATION OF UNNATURAL AMINO ACIDS

Peter SCHULTZ (Inventor), Lital Alfonta (Inventor), Jonathan R Chittuluru (Inventor), Alexander Deiters (Inventor), Dan Groff (Inventor), Daniel Summerer (Inventor), Meng-Lin Tsao (Inventor), Jiangyun Wang (Inventor), Ning Wu (Inventor), Jianming Xie (Inventor), Huaqiang. Zeng (Inventor)

Department of Biotechnology Engineering

Research output: Patent

Abstract

The invention relates to orthogonal pairs of tRNAs and cognate aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins contg. unnatural amino acids, and translation systems. In particular, the amino acid sequences and the encoding nucleotide sequences of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase and Escherichia coli leucyl-tRNA synthetase are disclosed. The synthetases were modified to accept a range of amino acid analogs as substrates for the charging of the tRNA. The tRNA were also modified to create a four- or five base anticodon that can be used to limit the incorporation of the foreign amino acid to specific sites. [on SciFinder(R)]

Original language	English
Patent number	19
State	Published - 2006

Keywords

Methanococcus Escherichia aminoacyl tRNA synthetas
unnatural amino acid orthogonal translation system

Cite this

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title = "ORTHOGONAL TRANSLATION COMPONENTS FOR THE IN VIVO INCORPORATION OF UNNATURAL AMINO ACIDS",

abstract = "The invention relates to orthogonal pairs of tRNAs and cognate aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins contg. unnatural amino acids, and translation systems. In particular, the amino acid sequences and the encoding nucleotide sequences of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase and Escherichia coli leucyl-tRNA synthetase are disclosed. The synthetases were modified to accept a range of amino acid analogs as substrates for the charging of the tRNA. The tRNA were also modified to create a four- or five base anticodon that can be used to limit the incorporation of the foreign amino acid to specific sites. [on SciFinder(R)]",

keywords = "Methanococcus Escherichia aminoacyl tRNA synthetas, unnatural amino acid orthogonal translation system",

author = "Peter SCHULTZ and Lital Alfonta and Chittuluru, {Jonathan R} and Alexander Deiters and Dan Groff and Daniel Summerer and Meng-Lin Tsao and Jiangyun Wang and Ning Wu and Jianming Xie and Huaqiang. Zeng",

year = "2006",

language = "English",

publisher = "The Scripps Research Institute, USA .",

type = "Patent",

note = "19",

}

TY - PAT

T1 - ORTHOGONAL TRANSLATION COMPONENTS FOR THE IN VIVO INCORPORATION OF UNNATURAL AMINO ACIDS

AU - SCHULTZ, Peter

AU - Alfonta, Lital

AU - Chittuluru, Jonathan R

AU - Deiters, Alexander

AU - Groff, Dan

AU - Summerer, Daniel

AU - Tsao, Meng-Lin

AU - Wang, Jiangyun

AU - Wu, Ning

AU - Xie, Jianming

AU - Zeng, Huaqiang.

PY - 2006

Y1 - 2006

N2 - The invention relates to orthogonal pairs of tRNAs and cognate aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins contg. unnatural amino acids, and translation systems. In particular, the amino acid sequences and the encoding nucleotide sequences of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase and Escherichia coli leucyl-tRNA synthetase are disclosed. The synthetases were modified to accept a range of amino acid analogs as substrates for the charging of the tRNA. The tRNA were also modified to create a four- or five base anticodon that can be used to limit the incorporation of the foreign amino acid to specific sites. [on SciFinder(R)]

AB - The invention relates to orthogonal pairs of tRNAs and cognate aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins contg. unnatural amino acids, and translation systems. In particular, the amino acid sequences and the encoding nucleotide sequences of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase and Escherichia coli leucyl-tRNA synthetase are disclosed. The synthetases were modified to accept a range of amino acid analogs as substrates for the charging of the tRNA. The tRNA were also modified to create a four- or five base anticodon that can be used to limit the incorporation of the foreign amino acid to specific sites. [on SciFinder(R)]

KW - Methanococcus Escherichia aminoacyl tRNA synthetas

KW - unnatural amino acid orthogonal translation system

UR - http://patentscope.wipo.int/search/en/WO2006110182

UR - https://www.mendeley.com/catalogue/aa41b71c-07e0-365d-a02b-237ba47c0a9c/

M3 - Patent

M1 - 19

PB - The Scripps Research Institute, USA .

ER -

ORTHOGONAL TRANSLATION COMPONENTS FOR THE IN VIVO INCORPORATION OF UNNATURAL AMINO ACIDS

Abstract

Keywords

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