Ovarian protein synthesis in the prawn Macrobrachium rosenbergii: Does ovarian vitellin synthesis exist?

Amir Sagi, Yoram Soroka, Evyatar Snir, Orna Chomsky, Yoram Milner, Jorge Calderon

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

During the reproductive cycle oocytes of Macrobrachium rosenbergii females grow in diameter from 20 µm to 650 µm, while the gonado-somatic index increases from 0.2 to 8.0. SDS-PAGE polypeptide profiles of the cytosolic fraction from ovaries in various stages were studied. Specific vitellin bands were identified by immunoblotting. In vitro synthesis of protein was measured by TCA precipitation and autoradiography of SDS-PAGE separated polypeptides. Incorporation of [35S]-methionine and cysteine was highest in animals having an oocyte diameter of 50-150 µm and lower in pre-vitellogenic ovaries (20-50 µm). The lowest synthesis was found in late-vitellogenic ovaries (300-500 µm). Most of the incorporation was found in non-vitellin polypeptide bands. These results suggest the existence of an extraovarian source of vitellogenin in M. rosenbergii.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalInvertebrate Reproduction and Development
Volume27
Issue number1
DOIs
StatePublished - 1 Jan 1995

Keywords

  • Decapoda
  • Macrobrachium rosenbergii
  • Ovarian polypeptides
  • Vitellin biosynthesis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

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