Over-expression of CarMT gene modulates the physiological performance and antioxidant defense system to provide tolerance against drought stress in Arabidopsis thaliana L

Arvind Kumar Dubey, Navin Kumar, Anil Kumar, Mohd Akram Ansari, Ruma Ranjan, Ambedkar Gautam, Meenakshi, Nayan Sahu, Vivek Pandey, Soumit Kumar Behera, Shekhar Mallick, Veena Pande, Indraneel Sanyal

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Drought is one of the major abiotic stresses which negatively affect plant growth and crop yield. Metallothionein (MTs) is a low molecular weight protein, mainly involved in metal homeostasis, while, its role in drought stress is still to be largely explored. The present study was aimed to investigate the role of MT gene against drought stress. The chickpea MT based on its up-regulation under drought stress was overexpressed in Arabidopsis thaliana to explore its role in mitigation of drought stress. The total transcript of MT gene was up to 30 fold higher in transgenic lines. Arabidopsis plants transformed with MT gene showed longer roots, better efficiency of survival and germination, larger siliques and higher biomass compared to WT. The physiological variables (A, WUE, G, E, qP and ETR) of WT plants were reduced during drought stress which recovered in transgenic Arabidopsis lines. The enzymatic and non-enzymatic antioxidant (APX, GPX, POD, GR, GRX, GST, CAT, MDHAR, ASc and GSH) levels were also enhanced in transgenic lines to provide tolerance. Simultaneously, drought responsive amino acids, i.e. proline and cysteine contents were higher in transgenic lines. Overall, the results suggest that MT gene is actively involved in the mitigation of drought stress and could be the choice for genetic engineering strategy to overcome drought stress.

Original languageEnglish
Pages (from-to)54-65
Number of pages12
JournalEcotoxicology and Environmental Safety
Volume171
DOIs
StatePublished - 30 Apr 2019
Externally publishedYes

Keywords

  • Arabidopsis
  • Chickpea
  • Drought
  • Metallothionein
  • Physiological performance
  • ROS

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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