Overcoming intrinsic restriction enzyme barriers enhances transformation efficiency in Arthrospira platensis C1

Wattana Jeamton, Sudarat Dulsawat, Morakot Tanticharoen, Avigad Vonshak, Supapon Cheevadhanarak

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The development of a reliable genetic transformation system for Arthrospira platensis has been a long-term goal, mainly for those trying either to improve its performance in large-scale cultivation systems or to enhance its value as food and feed additives. However, so far, most of the attempts to develop such a transformation system have had limited success. In this study, an efficient and stable transformation system for A. platensis C1 was successfully developed. Based on electroporation and transposon techniques, exogenous DNA could be transferred to and stably maintained in the A. platensis C1 genome. Most strains of Arthrospira possess strong restriction barriers, hampering the development of a gene transfer system for this group of cyanobacteria. By using a type I restriction inhibitor and liposomes to protect the DNA from nuclease digestion, the transformation efficiency was significantly improved. The transformants were able to grow on a selective medium for more than eight passages, and the transformed DNA could be detected from the stable transformants. We propose that the intrinsic endonuclease enzymes, particularly the type I restriction enzyme, in A. platensis C1 play an important role in the transformation efficiency of this industrial important cyanobacterium.

Original languageEnglish
Pages (from-to)822-830
Number of pages9
JournalPlant and Cell Physiology
Volume58
Issue number4
DOIs
StatePublished - 1 Apr 2017

Keywords

  • Arthrospira platensis
  • Cyanobacteria
  • Liposome
  • Nuclease
  • Transformation efficiency

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Fingerprint

Dive into the research topics of 'Overcoming intrinsic restriction enzyme barriers enhances transformation efficiency in Arthrospira platensis C1'. Together they form a unique fingerprint.

Cite this