Overexpression of rice glutaredoxin genes LOC_Os02g40500 and LOC_Os01g27140 regulate plant responses to drought stress

Anil Kumar, Arvind Kumar Dubey, Varun Kumar, Mohd Akram Ansari, Shiv Narayan, Meenakshi, Sanoj Kumar, Vivek Pandey, Pramod Arvind Shirke, Veena Pande, Indraneel Sanyal

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Glutaredoxins (Grxs) are small (10–15 kDa) glutathione (GSH) - dependent redox proteins. The role of Grxs are well documented in tolerance to heavy metal stress in prokaryotic and mammalian systems and a few plant genera, but is poorly understood in plants against drought. In the present study, two rice glutaredoxin (Osgrx) genes (LOC_Os02g40500 and LOC_Os01g27140) responsible for tolerance against heavy metal stress have been studied for investigating their role against drought. Each glutaredoxin gene was over-expressed in Arabidopsis thaliana to reveal their role in drought stress. The relative expression of both Osgrx genes was higher in the transgenic lines. Transgenic lines of both Osgrxs showed longer roots, higher seed germination, and survival efficiency during drought stress. The physiological parameters (PN, gs, E, WUE, qP, NPQ and ETR), antioxidant enzymes (GRX, GR, GPX, GST, APX, POD, SOD, CAT, DHAR, and MDHAR), antioxidant molecules (ascorbate and GSH) and stress-responsive amino acids (cysteine and proline) levels were additionally increased in transgenic lines of both Osgrxs to provide drought tolerance. The outcomes from this study strongly determined that each Osgrx gene participated in the moderation of drought and might be utilized in biological engineering strategies to overcome drought conditions in different crops.

Original languageEnglish
Article number110721
JournalEcotoxicology and Environmental Safety
Volume200
DOIs
StatePublished - 1 Sep 2020
Externally publishedYes

Keywords

  • Antioxidant
  • Arabidopsis thaliana
  • Drought
  • Glutaredoxin
  • Glutathione
  • ROS
  • Rice

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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