Oxidative stress in newborn erythrocytes

Yael Shahal, Erika R. Bauminger, Ehud Zmora, Miriam Katz, Dalia Mazor, Sarah Horn, Naomi Meyerstein

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Phenylhydrazine (PHZ) exposure is used to study in vitro red cell aging mechanisms dependent on Hb oxidation. The effect of PHZ on normal neonatal red blood cells was studied in unseparated blood and after separation into light and heavy cells. PHZ caused more extensive morphologic changes in neonatal than in adult red blood cells. PHZ exposure of neonatal cells caused less reduced glutathione depletion than in adult cells. Although we found the same total amount of oxidized Hb in both cells, a well-defined oxidation product of Hb was demonstrated by Mössbauer spectra only in neonatal cells. This oxidation product was not methemoglobin but a trivalent, high-spin iron compound. All neonatal cell populations were more sensitive to PHZ than were adult ones, as demonstrated by the presence of Heinz bodies at low PHZ concentration, which did not affect adult cells. These studies demonstrate greater sensitivity of neonatal cells to PHZ in all density-separated populations.

Original languageEnglish
Pages (from-to)119-122
Number of pages4
JournalPediatric Research
Issue number2
StatePublished - 1 Jan 1991

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health


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