Packaging of DNA origami in viral capsids: towards synthetic viruses

Stanislav Kler, Ran Zalk, Alexander Upcher, Idit Kopatz

Research output: Contribution to journalArticlepeer-review

Abstract

We report a new type of nanoparticle, consisting of a nucleic acid core (>7500 nt) folded into a 35 nm DNA origami sphere, encapsulated by a capsid composed of all three SV40 virus capsid proteins. Compared to the prototype reported previously, whose capsid consists of VP1 only, the new nanoparticle closely adopts the unique intracellular pathway of the native SV40, suggesting that the proteins of the synthetic capsid retain their native viral functionality. Some of the challenges in the design of such near-future composite drugs destined for gene delivery are discussed.

Original languageEnglish
Pages (from-to)11535-11542
Number of pages8
JournalNanoscale
Volume14
Issue number32
DOIs
StatePublished - 21 Jul 2022

ASJC Scopus subject areas

  • Materials Science (all)

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