Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy

Naru Yoneda; Xiangyu Quan; Manoj Kumar; Osamu Matoba

doi:10.1117/12.3007932

Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy

Naru Yoneda, Xiangyu Quan, Manoj Kumar, Osamu Matoba

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Two-photon holographic microscopy (TPHM) is a powerful technique for stimulating biological cells three-dimensionally. TPHM requires an imaging technique to detect fluorescence lights from the three-dimensionally distributed cells. In this study, parallel phase-shifting incoherent digital holography is applied to TPHM. Owing to the parallel phase-shifting method, the lights from the cells are detected with the frame rate of an image sensor. The feasibility of the proposed method is experimentally verified.

Original language	English
Title of host publication	Biomedical Imaging and Sensing Conference
Editors	Toyohiko Yatagai
Publisher	SPIE
ISBN (Electronic)	9781510663435
DOIs	https://doi.org/10.1117/12.3007932
State	Published - 1 Jan 2023
Externally published	Yes
Event	Biomedical Imaging and Sensing Conference 2023 - Yokohama, Japan Duration: 17 Apr 2023 → 21 Apr 2023

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	12608
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Biomedical Imaging and Sensing Conference 2023
Country/Territory	Japan
City	Yokohama
Period	17/04/23 → 21/04/23

Keywords

Incoherent holography
Two-photon microscopy

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.3007932

Cite this

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title = "Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy",

abstract = "Two-photon holographic microscopy (TPHM) is a powerful technique for stimulating biological cells three-dimensionally. TPHM requires an imaging technique to detect fluorescence lights from the three-dimensionally distributed cells. In this study, parallel phase-shifting incoherent digital holography is applied to TPHM. Owing to the parallel phase-shifting method, the lights from the cells are detected with the frame rate of an image sensor. The feasibility of the proposed method is experimentally verified.",

keywords = "Incoherent holography, Two-photon microscopy",

author = "Naru Yoneda and Xiangyu Quan and Manoj Kumar and Osamu Matoba",

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year = "2023",

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doi = "10.1117/12.3007932",

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Yoneda, N, Quan, X, Kumar, M & Matoba, O 2023, Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy. in T Yatagai (ed.), Biomedical Imaging and Sensing Conference., 1260818, Proceedings of SPIE - The International Society for Optical Engineering, vol. 12608, SPIE, Biomedical Imaging and Sensing Conference 2023, Yokohama, Japan, 17/04/23. https://doi.org/10.1117/12.3007932

Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy. / Yoneda, Naru; Quan, Xiangyu; Kumar, Manoj et al.
Biomedical Imaging and Sensing Conference. ed. / Toyohiko Yatagai. SPIE, 2023. 1260818 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 12608).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy

AU - Yoneda, Naru

AU - Quan, Xiangyu

AU - Kumar, Manoj

AU - Matoba, Osamu

PY - 2023/1/1

Y1 - 2023/1/1

N2 - Two-photon holographic microscopy (TPHM) is a powerful technique for stimulating biological cells three-dimensionally. TPHM requires an imaging technique to detect fluorescence lights from the three-dimensionally distributed cells. In this study, parallel phase-shifting incoherent digital holography is applied to TPHM. Owing to the parallel phase-shifting method, the lights from the cells are detected with the frame rate of an image sensor. The feasibility of the proposed method is experimentally verified.

AB - Two-photon holographic microscopy (TPHM) is a powerful technique for stimulating biological cells three-dimensionally. TPHM requires an imaging technique to detect fluorescence lights from the three-dimensionally distributed cells. In this study, parallel phase-shifting incoherent digital holography is applied to TPHM. Owing to the parallel phase-shifting method, the lights from the cells are detected with the frame rate of an image sensor. The feasibility of the proposed method is experimentally verified.

KW - Incoherent holography

KW - Two-photon microscopy

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U2 - 10.1117/12.3007932

DO - 10.1117/12.3007932

M3 - Conference contribution

AN - SCOPUS:85176119311

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Biomedical Imaging and Sensing Conference

A2 - Yatagai, Toyohiko

PB - SPIE

T2 - Biomedical Imaging and Sensing Conference 2023

Y2 - 17 April 2023 through 21 April 2023

ER -

Parallel phase-shifting incoherent digital holography for two-photon holographic microscopy

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this