Paraphysoderma sedebokerense GlnS III Is Essential for the Infection of Its Host Haematococcus lacustris

David Alors, Kevin R. Amses, Timothy Y. James, Sammy Boussiba, Aliza Zarka

Research output: Contribution to journalArticlepeer-review

Abstract

Glutamine synthetase (GlnS) is a key enzyme in nitrogen metabolism. We investigated the effect of the GlnS inhibitor glufosinate on the infection of H. lacustris by the blastocladialean fungus P. sedebokerense, assuming that interfering with the host nitrogen metabolism will affect the success of the parasite. Complete inhibition of infection, which could be bypassed by the GlnS product glutamine, was observed at millimolar concentrations of glufosinate. However, this effect of glufosinate was attributed to its direct interaction with the blastoclad and not the host, which results in development and growth inhibition of the blastoclad. In our P. sedebokerense draft genome, we found that the sequence of GlnS is related to another fungal GlnS, type III, found in many poor known phyla of fungi, including Blastocladiomycota and Chytridiomycota, and absent in the main subkingdom of fungi, the Dikarya. We further tested the ability of the blastoclad to utilize nitrate and ammonia as inorganic nitrogen sources and glutamine for growth. We found that P. sedebokerense equally use ammonia and glutamine and use also nitrate, but with less efficiency. Altogether, our results show that GlnS type III is mandatory for the development and growth of P. sedebokerense and could be an efficient target to develop strategies for the control of the fungal parasite of H. lacustris.

Original languageEnglish
Article number561
JournalJournal of Fungi
Volume8
Issue number6
DOIs
StatePublished - 1 Jun 2022

Keywords

  • Haematococcus lacustris
  • Paraphysoderma sedebokerense
  • fungi
  • glufosinate
  • glutamine synthetase
  • nitrogen metabolism

ASJC Scopus subject areas

  • Microbiology (medical)
  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Fingerprint

Dive into the research topics of 'Paraphysoderma sedebokerense GlnS III Is Essential for the Infection of Its Host Haematococcus lacustris'. Together they form a unique fingerprint.

Cite this