Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance

Narsingh R. Nirala; Shiju Abraham; Vinod Kumar; Shobhit A. Pandey; Umakant Yadav; Monika Srivastava; S. K. Srivastava; Vidya Nand Singh; Arvind M. Kayastha; Anchal Srivastava; Preeti S. Saxena

doi:10.1016/j.talanta.2015.05.059

Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance

Narsingh R. Nirala, Shiju Abraham, Vinod Kumar, Shobhit A. Pandey, Umakant Yadav, Monika Srivastava, S. K. Srivastava, Vidya Nand Singh, Arvind M. Kayastha, Anchal Srivastava, Preeti S. Saxena

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Abstract The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing. Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 μA/(mg/dL)/cm² and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL. Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode. This is evident from one order higher electron transfer rate constant (K_s) value in case of CH-prGO-AuNRs modified electrode (12.4×10^-2 cm/s), in contrast to CH-GO/ITO electrode (6×10^-3 cm/s). Additionally, very low K_m value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences. Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.

Original language	English
Article number	15651
Pages (from-to)	745-754
Number of pages	10
Journal	Talanta
Volume	144
DOIs	https://doi.org/10.1016/j.talanta.2015.05.059
State	Published - 20 Jul 2015
Externally published	Yes

Keywords

Chitosan
Composite
Electrochemical biosensors
Flavin adenine dinucleotide (FAD)
Glucose K
Gold nanorods
K
Partially reduced graphene oxide

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/j.talanta.2015.05.059

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@article{ccbcf13dba394ffb84de4bb132bfb997,

title = "Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance",

abstract = "Abstract The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing. Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 μA/(mg/dL)/cm2 and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL. Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode. This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10-2 cm/s), in contrast to CH-GO/ITO electrode (6×10-3 cm/s). Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences. Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.",

keywords = "Chitosan, Composite, Electrochemical biosensors, Flavin adenine dinucleotide (FAD), Glucose K, Gold nanorods, K, Partially reduced graphene oxide",

author = "Nirala, {Narsingh R.} and Shiju Abraham and Vinod Kumar and Pandey, {Shobhit A.} and Umakant Yadav and Monika Srivastava and Srivastava, {S. K.} and Singh, {Vidya Nand} and Kayastha, {Arvind M.} and Anchal Srivastava and Saxena, {Preeti S.}",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

year = "2015",

month = jul,

day = "20",

doi = "10.1016/j.talanta.2015.05.059",

language = "English",

volume = "144",

pages = "745--754",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier",

}

TY - JOUR

T1 - Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance

AU - Nirala, Narsingh R.

AU - Abraham, Shiju

AU - Kumar, Vinod

AU - Pandey, Shobhit A.

AU - Yadav, Umakant

AU - Srivastava, Monika

AU - Srivastava, S. K.

AU - Singh, Vidya Nand

AU - Kayastha, Arvind M.

AU - Srivastava, Anchal

AU - Saxena, Preeti S.

PY - 2015/7/20

Y1 - 2015/7/20

N2 - Abstract The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing. Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 μA/(mg/dL)/cm2 and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL. Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode. This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10-2 cm/s), in contrast to CH-GO/ITO electrode (6×10-3 cm/s). Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences. Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.

AB - Abstract The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing. Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 μA/(mg/dL)/cm2 and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL. Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode. This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10-2 cm/s), in contrast to CH-GO/ITO electrode (6×10-3 cm/s). Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences. Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.

KW - Chitosan

KW - Composite

KW - Electrochemical biosensors

KW - Flavin adenine dinucleotide (FAD)

KW - Glucose K

KW - Gold nanorods

KW - K

KW - Partially reduced graphene oxide

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U2 - 10.1016/j.talanta.2015.05.059

DO - 10.1016/j.talanta.2015.05.059

M3 - Article

C2 - 26452886

AN - SCOPUS:84937239061

SN - 0039-9140

VL - 144

SP - 745

EP - 754

JO - Talanta

JF - Talanta

M1 - 15651

ER -

Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance

Abstract

Keywords

ASJC Scopus subject areas

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