Partially reduced graphene oxide-gold nanorods composite based bioelectrode of improved sensing performance

Narsingh R. Nirala, Shiju Abraham, Vinod Kumar, Shobhit A. Pandey, Umakant Yadav, Monika Srivastava, S. K. Srivastava, Vidya Nand Singh, Arvind M. Kayastha, Anchal Srivastava, Preeti S. Saxena

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Abstract The present work proposes partially reduced graphene oxide-gold nanorods supported by chitosan (CH-prGO-AuNRs) as a potential bioelectrode material for enhanced glucose sensing. Developed on ITO substrate by immobilizing glucose oxidase on CH-prGO-AuNRs composite, these CH-prGO-AuNRs/ITO bioelectrodes demonstrate high sensitivity of 3.2 μA/(mg/dL)/cm2 and linear range of 25-200 mg/dL with an ability to detect as low as 14.5 mg/dL. Further, these CH-prGO-AuNRs/ITO based electrodes attest synergistiacally enhanced sensing properties when compared to simple graphene oxide based CH-GO/ITO electrode. This is evident from one order higher electron transfer rate constant (Ks) value in case of CH-prGO-AuNRs modified electrode (12.4×10-2 cm/s), in contrast to CH-GO/ITO electrode (6×10-3 cm/s). Additionally, very low Km value [15.4 mg/dL(0.85 mM)] ensures better binding affinity of enzyme to substrate which is desirable for good biosensor stability and resistance to environmental interferences. Hence, with better loading capacity, kinetics and stability, the proposed CH-prGO-AuNRs composite shows tremendous potential to detect several bio-analytes in the coming future.

Original languageEnglish
Article number15651
Pages (from-to)745-754
Number of pages10
JournalTalanta
Volume144
DOIs
StatePublished - 20 Jul 2015
Externally publishedYes

Keywords

  • Chitosan
  • Composite
  • Electrochemical biosensors
  • Flavin adenine dinucleotide (FAD)
  • Glucose K
  • Gold nanorods
  • K
  • Partially reduced graphene oxide

ASJC Scopus subject areas

  • Analytical Chemistry

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