PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins

Eitan Ben-Dov; Robert Manasherob; Arieh Zaritsky; Ze'ev Barak; Yoel Margalith

doi:10.1007/s0028403336

PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins

Eitan Ben-Dov, Robert Manasherob, Arieh Zaritsky, Ze'ev Barak, Yoel Margalith

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.

Original language	English
Pages (from-to)	96-99
Number of pages	4
Journal	Current Microbiology
Volume	42
Issue number	2
DOIs	https://doi.org/10.1007/s0028403336
State	Published - 1 Jan 2001

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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10.1007/s0028403336

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title = "PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins",

abstract = "An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.",

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AU - Margalith, Yoel

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AB - An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.

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PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins

Abstract

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