PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins

Eitan Ben-Dov, Robert Manasherob, Arieh Zaritsky, Ze'ev Barak, Yoel Margalith

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.

Original languageEnglish
Pages (from-to)96-99
Number of pages4
JournalCurrent Microbiology
Volume42
Issue number2
DOIs
StatePublished - 1 Jan 2001

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